针药结合对抑郁症睡眠障碍大鼠行为学及外侧缰核βCaMKⅡ蛋白表达的影响  

Effects of combination with acupuncture and western medicine on expression of βCaMKⅡ protein in lateral habenular nucleus and behavioristics of sleep deprivation depression rats

作　　者：俞裕天[1,2] 袁青[3] 陈津岩[4] 谢津津[1] 郎建英[5] 麦嘉玲[1] 曾晓林[1] 汪满霞 薛尔平 

机构地区：[1]广州中医药大学,广州510405 [2]佛山市禅城区中心医院治未病调养中心,佛山528031 [3]广州中医药大学针灸康复临床医学院,广州510405 [4]广州中医药大学核医学教研室,广州510405 [5]广州中医药大学基础医学院,广州510405

出　　处：《中华中医药杂志》2015年第5期1546-1550,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.81072878;No.81303041)~~

摘　　要：目的:探讨针药结合对抑郁症睡眠障碍(SDD)大鼠行为学变化及对外侧缰核(LHb)βCaMKⅡ蛋白表达的影响。方法:正常组8只SD大鼠常规合笼饲养,不接受任何刺激与处置。32只SD大鼠接受为期21d的孤养+CUMS法+REM睡眠剥夺法造模,其中28只顺利完成造模,再次分组为模型组、针刺组、药物组、针药组,每组各7只。模型组仅造模不干预;针刺组取印堂、神庭、配太冲(双)进行针刺,行导气同精法,各穴刺激15s,隔10min运针1次,每次留针20min;药物组给予舍曲林和阿普唑仑水溶液灌胃;针药组同时结合针刺组与药物组的两种干预方式;3组均连续干预7d。结果:造模的第7、14、21天,造模组大鼠与正常组大鼠比较,体质量、Open-Field得分、蔗糖偏好均出现显著的改变(P<0.05);第28天,各干预组体质量、Open-Field得分都较模型组有明显提升(P<0.05),针药组的Open-Field得分提升较针刺组和药物组均有统计学意义(P<0.05);仅针药组的蔗糖偏好较模型组得到了明显提升(P<0.05)且较针刺组和药物组有统计学意义(P<0.05);各干预组大鼠的LHbβCa MKⅡ蛋白表达均较模型组明显下调(P<0.05),针刺组与药物组效果相当,针药组明显优于针刺组和药物组(P<0.05)。结论:针刺干预对SDD有效,且与药物干预效果相当;针药结合则效果更优,值得在临床上加以进一步研究。Objective： To discuss the effect of combination with acupuncture and western medicine on expression of βCaMKⅡ protein in lateral habenular nucleus and behavioristics of sleep deprivation depression （SDD） rats. Methods： Eight SD rats in normal group were fed routinely without stimulation. Thirty-two SD rats were fed lonely and stimulated with chronic unpredictable mild stress （CUMS） and REM sleep deprivation. After the completion of animal model building 28 rats were left, and were randomly divided into 4 groups （model group, acupuncture therapy group, drug therapy group, and acupuncture combined with drug therapy group） with 7 rats in each group. Rats in model group received no treatment. Rats in acupuncture therapy group recieved acupuncture on Yintang （EX-HN3）, Shenting （DU24）, and Taichong （LR3） for 15s, and then stimulated acupoints every 10min and retained the needles for 20min every time. Rats in drug therapy group received intragastric administration with water solution of sertraline and alprazolam. Rats in acupuncture combined drug therapy group received stimulations of acupuncture and drug. All of the interventions were lasted for 7 days. Results： On the 7th day, 14th day and 21stday, the weight, open-field scores and sucrose preference of rats in model group were changed significantly （P〈0.05）. On the 28th day, the weight and open-field scores of rats in each intervention group were increased significantly compared with rats in normal group （P〈0.05）. Compared with acupuncture therapy group and drug therapy group, the open-field scores of rats in acupuncture combined drug therapy group were increased significantly （P〈0.05）. Compared with model group, acupuncture therapy group and drug therapy group, sucrose preference of rats in acupuncture combined drug therapy group was increased significantly （P〈0.05）. The expression of βCaMKⅡ in lateral habenular nucleus of rats in each intervention group was down-regulated significantly （P〈0.05）, and

关 键 词：抑郁症睡眠障碍 外侧缰核 β CaMKⅡ蛋白 针药结合 靳三针 

分 类 号：R277.7[医药卫生—中医学]