冠心康对ApoE^(-/-)小鼠骨髓源EPC生物学性能和SDF-1/CXCR4信号表达的影响  

Effects of Guanxinkangon on biological characteristics of bone marrow derived endothelial progenitor cells and signal expression of SDF-1/CXCR4 in Apo E^(-/-) mice

作　　者：秦合伟[1] 刘萍[1] 林圣超[2] 杜文婷[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《中华中医药杂志》2015年第5期1551-1555,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2012年度上海市优秀学术带头人计划项目(No.12XD1404700);教育部博士点基金资助项目(No.20133107110003);上海高级中西医结合人才培养项目(No.ZYSNXD012-RC-ZXY008)~~

摘　　要：目的:通过观察冠心康对骨髓内皮祖细胞(EPC)的增殖、迁移和黏附能力的影响,探知冠心康抗动脉粥样硬化(AS)的作用机制与SDF-1/CXCR4生物轴之间的关系。方法:36只8周龄雄性Apo E-/-小鼠分为模型对照组、AMD3100组和冠心康组;12只8周龄近交系C57BL/6/J小鼠作为正常对照组。各组于治疗12周时取材,采用HE染色检测各组小鼠主动脉横截面病理损伤程度;MTT比色法检测EPC增殖能力,Transwell迁移法检测迁移能力,EPC黏附实验检测EPC黏附能力;RT-PCR和Western blotting分别检测各组骨髓源EPC CXCR4 m RNA和蛋白表达。结果:冠心康可抑制骨髓源EPC的增殖、迁移、黏附,冠心康组小鼠骨髓源EPC的增殖、黏附和迁移能力明显低于模型对照组(P<0.05),冠心康组骨髓源EPC CXCR4 m RNA和蛋白表达水平明显低于模型对照组(P<0.01),冠心康组小鼠主动脉AS程度明显轻于模型对照组(P<0.05)。结论:中药复方冠心康可抑制骨髓源EPC的增殖、迁移、黏附,抑制骨髓源性EPC CXCR4基因和蛋白表达的作用,可能与冠心康调控SDF-1/CXCR4生物轴有关。Objective： To investigate the effects of Guanxinkang on proliferation, migration and adhesion ability of bone marrow derived endothelial progenitor cells （EPC）, so as to reveal the correlation between SDF-1/CXCR4 biological axis and anti-atherosclerosis function of Guanxinkang. Methods： Thrity-six male ApoE-/- mice were randomly divided into model group, AMD3100 group and Guanxinkang group, and 12 inbred line C57BL/6 mice were set as control group. Mice were sacrificed and tissues were collected on the 12th week. HE staining method was used to observe the pathological damage degree of aortic cross section of mice in each group. MTT method was used to test the proliferation ability of endothelial progenitor cells. Transfer ability was determined by using Transwell method, and adhesive capacity of EPC was determined by using endothelial progenitor cell adhesion experiments. RT-PCR and Western blotting were used to test mRNA and protein expression of CXCR4 in EPC. Results： Guanxinkang could inhibit the proliferation, migration and adhesion abilities of EPC, and the proliferation, migration and adhesion abilities of EPC in Guanxinkang group were lower than that of model group （P〈0.05）. The mRNA and protein expression of CXCR4 in EPC of Guanxinkang group was lower than that of model group （P〈0.01）, and the degree of aortic atherosclerosis of mice in Guanxinkang group was lighter than that of model group （P〈0.05）. Conclusion： Guanxinkang could inhibit proliferation, migration, adhesion and the gene and protein expression of CXCR4 of EPC, which might be related with the regulation effect of Guanxinkang on SDF-1/CXCR4 biological axis.

关 键 词：冠心康 骨髓源性内皮祖细胞 SDF-1/CXCR4生物轴 动脉粥样硬化 

分 类 号：R285.5[医药卫生—中药学]