桂芍知母汤及加味对佐剂性关节炎大鼠炎性因子及COX-2信号通路表达的影响  

Effects of Guishao Zhimu Decoction and its modification on expression of inflammatory factors and COX-2 signal pathway in adjuvant arthritis rats

作　　者：曲道炜[1] 朱辉[1] 杜斌[1] 杨北生[1] 艾华[1] 

机构地区：[1]辽宁中医药大学基础医学院,沈阳110032

出　　处：《中华中医药杂志》2015年第5期1719-1722,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：辽宁省科技厅博士启动基金项目(No.20121102);辽宁省教育厅科学研究一般项目(No.L2012352)~~

摘　　要：目的:探讨桂芍知母汤及加味对佐剂性关节炎(AA)大鼠炎性因子及环氧合酶-2(COX-2)通路表达的影响,并分析作用机制。方法:大鼠随机分为正常组、模型组、布洛芬组、桂芍知母汤组、桂芍知母加活血组(A)、桂芍知母加藤类组(B)。弗氏完全佐剂造模7d后给药3周,观察大鼠关节炎指数(AI)、足肿胀度(ER),ELISA法检测足跖软组织干扰素-γ(INF-γ)、白介素-4(IL-4)水平,免疫组化观察细胞核因子κB(NF-κB)、COX-2和血管内皮生长因子(VEGF)的表达。结果:桂芍知母汤及加味均能显著降低INF-γ,升高IL-4水平,显著减少关节滑膜NF-κB p65、COX-2、VEGF的蛋白表达(P<0.01)。结论:桂芍知母汤及活血、藤类加味方对AA大鼠足跖软组织及踝关节的肿胀和炎性有较好的缓解作用,其作用机制与调整炎性因子平衡,抑制COX-2通路蛋白表达有关。Objective： To observe the effects of Guishao Zhimu Decoction and its modification on expression of inflammatory factor and COX-2 pathway in adjuvant arthritis （AA） rat, and explore its mechanism. Methods： The rats were randomly divided into normal group, model group, Buluofen group, Guishao Zhimu Decoction group, modified Guishao Zhimu Decoction group （A）, and modified Guishao Zhimu Decoction group （B）. Seven days after modelling by Freund＇s complete adjuvant, after the 3-week treatment, the arthritis index （AI）, foot swelling ratio were observed, plantar soft tissue INF- γ, IL-4 were measured with ELISA method, protein expression of NF-κB, COX-2, VEGF were measured with immunohistochemical observation. Results： Soft tissue INF-γ was decreased, IL-4 was increased significantly in Guishao Zhimu Decoction and modified Guishao Zhimu Decoction groups, with significant reduced expression of NF-xB p65, COX-2, VEGF protein （P〈0.01）. Conclusion： Guishao Zhimu Decoction and modified Guishao Zhimu Decoction could alleviate the soft tissue and ankle symptoms of inflammation in AA rats. The mechanism maybe related to adjusting the balance of inflammatory factors, and inhibiting the COX-2 pathway protein expression.

关 键 词：桂芍知母汤 佐剂性关节炎 环氧合酶2 细胞核因子-ΚB 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]