活血四妙汤对高尿酸血症大鼠早期肾损害肾组织CD40表达的影响  

Effects of Huoxue Simiao Decoction on renal tissue and CD40 expression in the hyperuricemia rats of early renal damage

作　　者：尉万春 李伟[2] 刘文毅[2] 李根茂[3] 葛东宇[3] 邱模炎[1] 

机构地区：[1]中国中医科学院望京医院,北京100102 [2]北京中医药大学第三附属医院,北京100029 [3]北京中医药大学基础科研中心,北京100029

出　　处：《中华中医药杂志》2015年第5期1748-1752,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京中医药大学自主选题项目(No.2013-JYBZZ-JS-188)~~

摘　　要：目的:观察活血四妙汤对高尿酸血症(HUA)大鼠早期肾损害的肾组织及其CD40表达的影响,探讨其防治HUA肾损害早期病变的机制。方法:采用饲喂腺嘌呤和盐酸乙胺丁醇建立HUA早期肾损害模型。SD雄性大鼠随机分为正常组、模型组、别嘌醇组及活血四妙汤小、中、大剂量组,于造模并给药后14d采血并取材。采用比色法检测血尿酸、血肌酐值,应用HE,Masson等方法进行切片染色,应用免疫组化检测CD40的表达。结果:模型组与正常组相比,血尿酸值显著增高(P<0.05),CD40表达增多(P<0.05),活血四妙汤大剂量、中剂量组与模型组相比,血尿酸值显著下降(P<0.05),活血四妙汤小剂量组、中剂量组、大剂量组CD40表达与模型组相比,显著下降(P<0.05),且在一定范围内,呈量效关系。结论:活血四妙汤可以降低HUA大鼠血尿酸值,并且可以延缓尿酸性肾损害,其机制可能是通过降低炎性因子CD40的表达延缓HUA大鼠早期肾损害。Objective： To observe the Huoxue Simiao Decoction on renal tissue and CD40 expression in the hyperuricemia rats of early renal damage, and to explore its mechanism of prevention and treatment. Methods： The byperuricemia rat model of early renal damage was established by being gavaged with adenine and ethambutol. SD male rats were randomly divided into six groups： normal group, model group, allopurinol group, Huoxue Simiao Decoction low, middle and high dose groups. After 14 days of administration, bllood and material were collected. Uric acid and serum creatinine were respectively detected by colorimetric method, and HE staining, Masson staining, immunohistochemistry detection of renal CD40 were all measured. Results： Compared with normal group, the uric acid of model group was significantly higher （P〈0.05）, and the expression of CD40 was increased （P〈0.05）, the uric acid of Huoxue Simiao Decoction high and middle dose groups was significantly decreased （P〈0.05）, the expression of CD40 of Huoxue Simiao Decoction low, middle and high dose groups were decreased significantly compared with the model group （P〈0.05）, and the greater the dose, the better the outcome. Conclusion： Huoxue Simiao Decoction could regulate uric acid in rats with hyperuricemia. Its efficacy is associated with the decrease of CD40 expression.

关 键 词：活血四妙汤 高尿酸血症 早期肾损害 HE染色 MASSON染色 CD40 

分 类 号：R285.5[医药卫生—中药学]