HPLC测定不同产地巴戟天中5种茜草素型蒽醌的含量  

Determination of Five Active Components in Morinda officinalis from Different Habitats by HPLC

作　　者：史辑[1] 刘梓晗[1] 王玲[1] 贾天柱[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中药材》2015年第2期245-248,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金青年基金项目(81001635);国家中医药管理局行业专项(201107007)

摘　　要：目的：建立用HPLC同时测定不同产地巴戟天中5种茜草素型蒽醌含量的方法。方法：采用HPLC测定巴戟天中1-甲氧基-2-羟基蒽醌、1,2-二甲氧基-3-羟基蒽醌、甲基异茜草素-1-甲醚、1,3-二羟基-2-甲氧基蒽醌、甲基异茜草素的含量。色谱条件：Ecosil C18色谱柱（250 mm×4.6 mm,5μm）,流动相：乙腈-0.2%磷酸梯度洗脱,流速：0.8 m L/min,检测波长：277 nm,柱温：30℃。结果：1-甲氧基-2-羟基蒽醌、1,2-二甲氧基-3-羟基蒽醌、甲基异茜草素-1-甲醚、1,3-二羟基-2-甲氧基蒽醌、甲基异茜草素5种蒽醌质量浓度分别在0.2856~34.27μg/m L、0.3268~39.22μg/m L、0.3450~41.40μg/m L、0.1248~14.98μg/m L、0.0508~6.096μg/m L范围内与峰面积呈良好的线性关系,加样回收率分别为99.4%、100.2%、101.4%、97.2%、103.2%（RSD均小于3%）。对10个不同产地的巴戟天样品进行测定,1-甲氧基-2-羟基蒽醌、1,2-二甲氧基-3-羟基蒽醌、甲基异茜草素-1-甲醚、1,3-二羟基-2-甲氧基蒽醌、甲基异茜草素的含量变化范围为0.0025~0.0722 mg/g、0.0016~0.0658 mg/g、0.0022~0.0684 mg/g、0.0182~0.3965 mg/g、0.0014~0.0179 mg/g。结论：该方法准确、可靠,可同时测定巴戟天中5种茜草素型蒽醌成分的含量,为评价不同产地巴戟天质量提供了科学依据。Objective ：To establish a sensitive and specific high performance liquid chromatography（HPLC） method for simultane- ous determination of alizarin anthraquinones （ 1 -methoxy-2-hydroxy anthraquinone, 1,2-dimethoxy-3-hydroxy anthraquinone, rubiadin-1 - methylether, 1,3-dihydroxy-2-methoxy anthraquinone, rubiadin）in Morinda officinalis from different habitats. Methods：The analysis was carried out on an Ecosil C18 column （250 mm × 4. 6 mm,5 μm）and detected with an UV detector at the wavelength of 277 nm. Gradient elution was carried out with acetonitrile-O. 2% phosphoric acid at the flow rate of 0. 8 mL/min. The column temperature was set at 30 ℃. Results ： The calibration curves were linear in the range of 0. 2856 - 34. 27 μg/mL for 1-methoxy-2-hydroxy anthraquinone （ r = 0. 9999） ,0. 3268 ~ 39. 22 μg/mL for 1,2-dimethoxy-3-hydroxy anthraquinone （ r = 0. 9999 ） ,0. 3450 - 41.40 μg/mL for rubiadin-1- methylether（ r = 0. 9999 ） ,0. 1248 ~ 14. 98 μg/mL for 1,3-dihydroxy-2-methoxy anthraquinone（ r = 0. 9999 ） and 0. 0508 - 6. 096 μg/ mL for rubiadin（ r = 0. 9994）, respectively. The average recoveries of the five components were 99.4%, 100. 2%, 101.4% ,97. 2%, 103.2% , respectively. The content of active components of samples from 10 different hatitats were in the range of 0. 0025 - 0. 0722 mg/ g,0. 0016 ~0. 0658 mg/g,0. 0022 - 0. 0684 mg/g,0. 0182 ~ 0. 3965 mg/g and 0. 0014 ~ 0. 0179 mg/g. Conclusion： The established method is accurate,reliable,and can be used for the simultaneous determination of the five components in Morinda officinalis, which provides a scientific basis for the quality evaluation of Morinda officinalis.

关 键 词：巴戟天 含量测定 HPLC 

分 类 号：R284.1[医药卫生—中药学]