橘皮汤对顺铂致水貂呕吐模型的作用研究  

Effect of Ju-Pi-Tang on Cisplatin-induced Emetic Model in Minks

作　　者：刘冉冉[1] 杨志宏[1] 杨玉玲[2] 刘占涛[1] 岳旺[1] 

机构地区：[1]青岛大学药学院,山东青岛266021 [2]青岛大学附属医院感染科,山东青岛266003

出　　处：《中药材》2015年第2期339-343,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(30873108;81273702);山东省卫生厅项目(2013WS0259)

摘　　要：目的：研究《金匮要略》中橘皮汤对顺铂所致水貂呕吐模型的止呕作用及对外周和中枢c-fos和P物质表达的影响。方法：将水貂随机分成正常对照组、橘皮汤空白组、模型组、昂丹司琼阳性对照组和阿瑞吡坦阳性对照组及橘皮汤高、中、低剂量组,各组均于注射顺铂前1天开始给药,连续给药4 d,观察注射顺铂后0~72 h各组水貂的干呕和呕吐次数,并用免疫组化方法观察水貂回肠和脑组织c-fos、P物质的表达。结果：与模型组比较,整个观察期内,橘皮汤,尤其是高、中剂量,能减少顺铂所致水貂的干呕和呕吐次数;与阳性药比较,0~24 h急性期,橘皮汤高剂量组对水貂干呕次数的减少优于阿瑞吡坦组,24~72 h延迟期橘皮汤高剂量组对水貂干呕和呕吐次数的减少优于昂丹司琼组。橘皮汤各组回肠和脑组织c-fos、P物质阳性表达颗粒的灰度值均高于模型组。结论：橘皮汤对顺铂所致水貂呕吐模型具有长效止呕作用。Objective：To study the antiemetic effect of Ju-Pi-Tang from Jin Kui Yao Lue on cisplatin-induced emetic model in minks, and to observe the immunoexpression of peripheral and central c-los and substance P. Methods ： The minks were randomly divided into blank control group, Ju-Pi-Tang blank control group, model group, ondansetron group, aprepitant group, Ju-Pi-Tang（ in high-, mid-, and low-dose）groups. Every group was administered with the antiemetic agent or distilled water on 24 h before cisplatin injection. The antiemetic effect of drugs was investigated in the emetic model of minks induced by cisplatin in 72 h observation. Immunohistochemistry was used to compare the differences of c-fos and substance P expression in the area postrema of brain and distal ileum tissues. Results ： During observation period, compared with model group, the frequency cisplatin induced retching and vomiting was significantly reduced by Ju-Pi-Tang in high- and mid-dose groups, during the 0 - 24 h acute period, the number of retching of Ju-Pi-Tang in high-dose group was decreased more than aprepitant group, during the 24 - 72 h delayed period, the number of both retching and vomiting was decreased more than ondansetron group, after 72 h of cisplatin administration, compared with model group, the grey levels of c-fos and substance P expression in distal ileum and brain tissues of Ju-Pi-Tang groups were higher significantly. Conclusion：Ju-Pi-Tang has a good effect a- gainst cisplatin-induced emesis in minks.

关 键 词：橘皮汤 顺铂 水貂 止呕 C-FOS P物质 

分 类 号：R285.5[医药卫生—中药学]