薏苡仁水溶性蛋白提取工艺的优化  

Study on Optimum Extraction Process of Water Soluble Protein from Coicis Semen

作　　者：武皓[1] 巩丽丽[2] 杨勇[3] 容蓉[1] 鞠建庆[3] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学实验中心,山东济南250355 [3]山东中医药大学基础医学院,山东济南250355

出　　处：《中药材》2015年第2期376-380,共5页Journal of Chinese Medicinal Materials

基　　金：国家重点基础研究发展计划973计划课题(2013CB5318000)

摘　　要：目的:优化薏苡仁水溶性蛋白提取工艺。方法:用半胱氨酸盐酸盐溶液在碱性条件下提取薏苡仁蛋白,对影响蛋白提取的因素进行单因素考察。结合单因素考察结果,选取浸提温度、浸提p H、料液比、浸提时间设计4因素3水平的正交试验,分析各因素对结果的影响及因素间交互作用。结果:薏苡仁水溶性蛋白最佳提取工艺为:浸提温度40℃,浸提p H 10,料液比1∶20,浸提时间4 h。温度与料液比、温度与时间的交互作用会降低蛋白浸提率。结论:优化后的提取工艺经济简单,稳定可行。Objective ：To optimize the extraction process of water soluble protein from Coicis Semen. Methods ： Coicis Semen was used to extract protein with cysteine hydrochloride aqueous solution in alkaline environment. The single factor test was conducted with the factors of extraction temperature, extraction pH, solid-liquid ratio and extraction time. In combination with single factor test results, a 4 factors 3 levels orthogonal experiment was designed with the factors of extraction temperature, extraction pH, solid-liquid ratio and extraction time. The effect of various factors on the results and the interactions were analyzed by range analysis, variance analysis and partial least squares regression analysis. Resuhs ： The optimum extraction process of water soluble protein from Coicis Semen was as fol- lows ：the extraction temperature was 40 ℃, the extraction pH was 10, the solid-liquid ratio was 1：20 and the extraction time was 4 h. The interaction of temperature and solid-liquid ratio, as well as temperature and extraction time would lower protein extraction rate. Conclusion ：The optimized extraction process is economical, simple, reasonable and practicable.

关 键 词：薏苡仁蛋白 提取工艺 正交试验 偏最小二乘回归分析 

分 类 号：R284.2[医药卫生—中药学]