桑赤锈病病原菌拮抗微生物的分离与筛选  

Isolation and Screening of Antagonistic Microorganisms Against Pathogenic Bacteria of Mulberry Red Rust

作　　者：崔萍[1] 安慧[1] 李美娜[1] 

机构地区：[1]河池学院化学与生物工程学院,广西宜州546300

出　　处：《贵州农业科学》2015年第4期82-84,共3页Guizhou Agricultural Sciences

基　　金：广西教育厅面上项目"广西桑赤锈病的生物防治研究"(桂教科研200911MS215)

摘　　要：为寻求生防微生物用于防治桑赤锈病,采用平板对峙法初步筛选农田根际及非根际土壤、贫瘠山地土壤及健康桑叶分离的微生物,用琼脂平板扩散法测定初筛拮抗菌株发酵液的抑菌活性。结果表明:初筛共得到25株菌株对桑赤锈病病原菌有明显的抑菌带,复筛共得到5株菌株对其具有较强的拮抗作用,以细菌C53的抑菌效果最好,抑菌率达65.4%。Microorganisms isolated from farmland rhizosphere and non-rhizosphere soil,rhizosphere soil of plants grown in infertile mountainous area and healthy mulberry leaves were preliminarily screened by the agar plate method,and then the bacteriostatic activity of fermentation broth of screened antagonistic strains was determined by the agar plate diffusion method to probe biological control of mulberry red rust. The results showed that 25 preliminarily screened strains have obvious inhibition zones against mulberry red rust and five secondarily screened strains have the stronger antagonism against mulberry red rust.The antibacterial rate of C53 strain can be up to 65.4%.

关 键 词：桑赤锈病 病原菌 微生物 生物防治 拮抗作用 

分 类 号：S888.71[农业科学—特种经济动物饲养]