贵州蔗区甘蔗黄叶病发生情况的分子鉴定  

Molecular Identification of Sugarcane Yellow Leaf Virus in Sugarcane Field in Guizhou

作　　者：付瑜华[1] 周玉飞[1] 张正学[1] 雷石富[1] 李向勇[1] 卢加举[1] 

机构地区：[1]贵州省亚热带作物研究所,贵州兴义562400

出　　处：《贵州农业科学》2015年第4期85-87,91,共4页Guizhou Agricultural Sciences

基　　金：贵州省科学技术基金项目"果蔗NBS-LRR类抗病基金同源序列的克隆与分析"[黔科合J字(2012)2253];贵州省科技厅农业攻关项目"强宿根抗旱甘蔗新品种的培育及应用"[黔科合NY字(2012)3034];贵州省农业科学院专项"黔蔗吨糖田关键技术研究与示范"[黔农科院院专项(2012003)];贵州省农业科学院基金项目"蔗地绿肥压青对土壤改良及抗旱效应的研究"[黔农科合(基金)2011015]

摘　　要：为明确甘蔗黄叶病在贵州各蔗区的发生情况,对贵州甘蔗生产主产区的23个甘蔗疑似感病植株进行了甘蔗黄叶病的分子检测。结果表明:8个甘蔗生产区中6个蔗区检测到甘蔗黄叶病毒(SCYLV),样本阳性检测率为47.8%。结论:甘蔗黄叶病在贵州多个蔗区均有发生,不同品种间的感病情况存在差异,生产上需根据实际情况采取相应的防治措施。In order to determine the occurrence of sugarcane yellow leaf disease in Guizhou,23 sugarcane varieties with disease symptom from eight sugarcane fields were detected for sugarcane yellow leaf virus by RT-PCR.Results：varieties from six sugarcane fields were infected with the infection incidence of 47.8%.Conclusion：sugarcane yellow leaf disease existed in multiple sugarcane fields of Guizhou and the infection situation varied among varieties,which means that corresponding preventive measures should be adopted based on the actual situation.

关 键 词：甘蔗黄叶病 分子检测 RT-PCR 贵州 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]