层层自组装血管内皮生长因子/磺化壳聚糖改性钛表面的研究  

Surface Modification of Titanium with Vascular Endothelial Growth Factor/Sulfated Chitosan by Use of Layer-by-layer Self-assembling Technique

作　　者：汪洋[1] 刘小斌[1] 叶巍[1] 梅黎[1] 魏战杰[1] 张凯伦[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心血管外科,武汉430022

出　　处：《华中科技大学学报（医学版）》2015年第2期138-142,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(No.2013CFB166)

摘　　要：目的利用层层自组装技术,在钛金属表面构建血管内皮生长因子/磺化壳聚糖(VEGF/SCS)多层膜,评价其体外血液相容性,并探讨其在医用金属表面改性方面的应用前景。方法采用化学合成制备磺化壳聚糖(sulfated chitosan,SCS)并对其表征进行分析;利用层层自组装技术在钛表面构建VEGF/SCS多层膜;对钛表面涂层进行表征分析并评价其体外血液相容性。结果钛-VEGF/SCS自组装多层膜表面光整,该涂层体外溶血率为0.567%,可延长部分活化凝血酶原时间(APTT)至(49.29±1.05)s,减少血小板粘附和激活。结论 VEGF/SCS层层自组装形成的多层膜可以提高钛合金的体外血液相容性,显示其在医用金属表面改性方面的应用前景。Objective To establish the multilayers of vascular endothelial growth factor/sulfated chitosan（VEGF/SCS）on the surface of titanium by layer-by-layer（LBL）self-assembling technique,and to explore the in vitro blood compatibility of this titanium and its application prospect in the area of medical metal surface modification. Methods Sulfated chitosan was prepared by chemical synthesis and its characteristics were analyzed. The VEGF/SCS multilayers were obtained by LBL self-assembling technique. The physical properties of the multilayers were analyzed. The blood compatibility of the films in vitro was evaluated. Results The surface of VEGF/SCS multilayers was smooth and uniform. The rate of hemolysis of VEGF/SCS multilayers was 0.567 %, and VEGF/SCS multillayers could prolong APTT to （49.29±1.05） s, inhibit platelet adhesion and activation ef feetively. Conclusion The in vitro blood compatibility of titanium alloy can be improved significantly after being coated with VEGF/SCS multilayers,and this technology shows favorable prospects in the area of medical metal surface modification.

关 键 词：钛 磺化壳聚糖 自组装 血液相容性 

分 类 号：R318.11[医药卫生—生物医学工程]