SATB1及wnt/β-catenin信号通路相关分子在子痫前期胎盘组织中的表达及意义  

Expression and significance of SATB1 and wnt/β-catenin signaling molecule in the placenta of preeclampsia

作　　者：庄白妹 罗欣[1] 饶海英[1] 李庆姝[1] 刘西茹[1] 漆洪波[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,400016

出　　处：《中华妇产科杂志》2015年第4期283-290,共8页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金（81300509,81300508）;国家重点临床专科建设项目（201101CKZD）;广东省产科重大疾病重点实验室专项基金（粤科财字2011年102号文）

摘　　要：目的：探讨富含AT序列的特异性结合蛋白1（SATB1）及蛋白（wnt）/β连环蛋白（β-catenin）信号通路相关分子在调控滋养细胞侵袭以及在子痫前期发病中的作用。方法收集2013年3月-2014年3月在重庆医科大学附属第一医院行人工流产术的20例孕8-10周孕妇的绒毛组织（早孕组）；同期在妇产科住院行水囊引产的18例中孕期（孕18-20周）孕妇的胎盘组织（中孕组）；同期行择期剖宫产术的20例健康足月妊娠孕妇的胎盘组织（正常足月组）；同期住院行选择性剖宫产术分娩的20例子痫前期孕妇（孕33-38周）的胎盘组织（子痫前期组）。采用免疫组化SP法检测各组绒毛或胎盘组织中SATB1及β-catenin的蛋白表达定位；采用蛋白印迹法检测各组绒毛或胎盘组织中SATB1及β-catenin的蛋白表达水平；采用细胞免疫荧光法检测SATB1及β-catenin在滋养细胞株HTR8/SVneo细胞中的定位表达，并对细胞中SATB1及β-catenin蛋白表达水平进行检测；采用免疫共沉淀法检测子痫前期组胎盘组织和缺氧/复氧组HTR8/SVneo细胞中的SATB1与β-catenin的相互关系；采用明胶酶谱法检测子痫前期组和缺氧/复氧组细胞中基质金属蛋白酶（MMP）2、9的活性水平。结果（1）正常足月组胎盘组织合体滋养细胞结节和纤维素样坏死很少见，毛细血管数目丰富；子痫前期组胎盘组织可见合体滋养细胞胞核空泡化明显，有较多纤维素样坏死，绒毛内微血管数目减少。（2）各组绒毛或胎盘组织中均可见SATB1棕黄色染色颗粒，子痫前期组胎盘组织中SATB1阳性染色强度较正常足月组明显减弱。（3）各组绒毛或胎盘组织中均可见β-catenin蛋白的表达，子痫前期组胎盘组织中β-catenin阳性染色强度较正常足月组减弱。（4）早孕组、中孕组、正常足月组及子痫前期组中SATB1蛋白表达水平分别为0.300±0.009、0.271±0.015、0.238±0.018及0.1Objective To explore the role of specific AT rich sequence binding protein 1（SATB1） and wnt/β-catenin signaling pathways in the regulation of trophoblast invasion and its effect in the pathogenesis of preeclampsia. Methods From March 2013 to March 2014, 20 cases of human villous tissues （early pregnancy group） from women of 8-10 gestational weeks who received artificial abortion at the First Affiliated Hospital of Chongqing Medical University, 18 cases of placental tissues （mid-pregnancy group） from women of 18-20 gestational weeks who had labor induction by water bag, 20 cases of placental tissues （normal full-term group） from healthy full-term pregnancy women and 20 cases of placental tissues （preeclamptic group） from women with preeclampsia who received elective c-section in were collected. Immunohistochemical SP method was utilized to determine the position of SATB1 and beta-catenin in villous tissues or placental tissues. Western blot was performed to analyze the expression level of SATB1 and beta-catenin in villous tissues or placental tissues. Immunofluorescence assay was used to determine the location of SATB1 andβ-catenin in HTR8/SVneo cells. Western blot was performed to detect the expression level of SATB1 and beta-catenin in HTR8/SVneo cells cultured in normoxia and hypoxia reoxygenation（H/R） condition. Co-Immunoprecipitation detection was used to evaluate the interaction between SATB1 andβ-catenin in placental tissues in preeclamptic group and HTR8/SVneo cells in H/R group. Gelatin zymography analysis was used to measure the activity of matrix metalloproteinases（MMP）-2 and 9 in placental tissues from preeclamptic group and HTR8/SVneo cells in H/R group. Results （1） In the normal full-term group, rare syncytiotrophoblastic nodule, less fibrinoid necrosis and abundant numbers of capillary could be observed in placental tissues. In comparison, there were obvious vacuolation in the cytoblast of syncytiotrophoblast, rich fibrinoid necrosis and poor numbers of villous cap

关 键 词：先兆子痫 胎盘 核基质附着区结合蛋白质类 WNT蛋白质类 Β连环素 基质金属蛋白酶2 基质金属蛋白酶9 

分 类 号：R714.244[医药卫生—妇产科学]