利用^(31)P NMR谱快速测定药物中ATP、ADP、AMP以及磷酸盐的含量  

Rapid determination of ATP, ADP, AMP and phosphate in drug by ^(31)P NMR spectroscopy

作　　者：李添宝[1] 罗敬[1] 尹帮少[1] 

机构地区：[1]湖南师范大学化学化工学院,湖南长沙410081

出　　处：《药学学报》2015年第5期583-586,共4页Acta Pharmaceutica Sinica

摘　　要：利用31P NMR谱对同一样品中ATP、ADP、AMP以及磷酸钠和焦磷酸钠等含磷物质进行定性定量分析,得到ATP、ADP、AMP在0.004~0.080 mol·L-1,Na H2PO4、Na4P2O7在0.005~0.100 mol·L-1内具有很好的线性关系。该方法精密度为0.40%~1.30%,添加回收率为96.9%~105.2%。把该方法应用于ATP注射液的测定,测得ATP注射液中的杂质为ADP和磷酸盐,ATP的含量符合药典要求。实验结果表明该方法重现性好、准确度高,而且无需前处理、操作简单、快速、无其他元素的干扰,是一种全新、可靠的分析ATP、ADP、AMP以及磷酸盐的方法。The content of ATP, ADP, AMP, sodium phosphate and sodium pyrophosphate were determined by 31p NMR, the linear range of ATP, ADP and AMP were found to be 0.004-0.080 mol.L-1, sodium phosphate and sodium pyrophosphate were 0.005-0.100 mol.L-1. The RSD were 0.40%-1.30%, the recovery were 96.9%- 105.2%. The method has been applied to the determination of ATP injection. The impurities of ATP injection were ADP and sodium phosphate. The content of ATP is in line with the requirement of the pharmacopoeia. The results indicated that the method is of good reproducibility, high accuracy, rapid and simple operation, without pretreatment and interference of other elements, 31p NMR is a new and reliable method of analyzing ATP, ADP, AMP and phosphate.

关 键 词：31P NMR谱 ATP ADP AMP 磷酸钠 焦磷酸钠 杂质 

分 类 号：R917[医药卫生—药物分析学]