水牛角中水溶性物质化学组成分析与鉴定  

Analysis and identification of water soluble components of water buffalo horn

作　　者：刘睿[1,2,3] 段金廒[1,3] 吴皓[1,2] 刘培[1,3] 尚尔鑫[1,3] 钱大玮[1,3] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,江苏南京210023 [2]f南京中医药大学江苏省海洋药用生物资源研究与开发重点实验室,江苏南京210023 [3]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210023

出　　处：《药学学报》2015年第5期594-598,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81202861,81274017);教育部博士点基金资助项目(20113237120011);江苏高校优势学科建设工程资助项目(PAPD);2014国家中医药行业科研专项(201407002)

摘　　要：系统研究水牛角中水溶性物质的化学组成,建立水牛角中水溶性物质分析与鉴定的方法。采用shotgun蛋白质鉴定技术研究水牛角中蛋白质类物质组成,主要为角蛋白、胶原蛋白、桥粒蛋白等结构蛋白;以超滤系统与LC-MS/MS结合鉴定了水牛角中肽类物质组成,主要为源于角蛋白与胶原蛋白等蛋白质的C末端肽段与非C末端肽段;通过与对照品比对,确定了水牛角中次黄嘌呤、尿苷、鸟苷及腺苷4种核苷类物质。本文研究思路可用于角类动物药中水溶性物质组成与分析,为揭示角类动物药功效物质基础提供依据。This study is to analyze and identify the water soluble components of water buffalo horn （Bubali Cornu, WBH）, and also establish a method for investigating these components. Shotgun proteomic analysis identified proteins in WBH aqueous extraction： keratin, collagen, desmoglein, etc. Ultrafiltration and LC-MS/MS were used to separate and identify the peptides in WBH aqueous extract, as a result, identified peptides were mainly derived from nonspecific degradation products of keratin and collagen, which including C-terminal peptides and non C-terminal peptides. Hypoxanthine, uridine, guanosine, and adenosine were identified by comparing with the standards. The strategy in present study could be used in analyzing water soluble components of animal horn derived TCM. It provides a reference for investigation of the material basis of animal horn derived TCM.

关 键 词：水牛角 蛋白质 肽类 

分 类 号：R917[医药卫生—药物分析学]