抗脑抗体对创伤性脑损伤后血脑屏障和脑水肿的影响  

Effect of antibrain-antibody on blood-brain barrier and cerebral edema after traumatic brain injury

作　　者：苏海[1] 张毅[1] 苏祖禄[1] 陈皓[1] 

机构地区：[1]重庆医科大学附属永川医院神经外科,402160

出　　处：《中华创伤杂志》2015年第5期467-470,共4页Chinese Journal of Trauma

基　　金：重庆市永川区科委基金资助项目（2010075）

摘　　要：目的 探讨兔创伤性脑损伤（TBI）后血清抗脑抗体（AB-Ab）水平的变化及其对血脑屏障（BBB）和脑水肿的影响. 方法 将45只新西兰兔按随机数字表法分为对照组（5只）、假手术组（20只）和TBI组（20只）,TBI组又分为1,3,7,14 d亚组.假手术组只开骨窗不打击;TBI组建立脑单侧重型液压冲击TBI模型.分别于术后1,3,7,14d检测各组血清AB-Ab浓度、BBB通透性和脑含水量. 结果 假手术组、TBI组血清AB-Ab含量、BBB通透性和脑含水量在各时相点均高于对照组（P＜0.05）,TBI组血清AB-Ab含量、BBB通透性和脑含水量在3,7,14 d均高于假手术组（P＜0.05）. 结论 TBI后血清中AB-Ab与BBB通透性升高和脑水肿程度呈同步性改变,提示AB-Ab可能是BBB通透性升高和脑水肿的原因之一.Objective To investigate the changes of antibrain-antibody （AB-Ab） in blood serum and the effect of AB-Ab on blood-brain barrier （BBB） and cerebral edema after the traumatic brain injury （TBI） in rabbits.Methods Forty-five New Zealand rabbits were randomly divided into control group （n =5）,sham operation group （fenestration only without injury,n =15） and TBI group （severe lateral fluid percussion brain injury,n =15） according to the random number table.Sham and TBI animals were subdivided at postoperative 1,3,7 and 14 days.Level of AB-Ab,BBB permeability and brain water content were determined after operation.Results At each time point,serum AB-Ab level,BBB permeability and brain water content were higher in sham operation group and TBI group than in control group （P 〈0.05）.At postoperative 3,7 and 14 days,serum AB-Ab level,BBB permeability and brain water content were higher in TBI group than in sham operation group （P 〈 0.05）.Conclusion Serum level of AB-Ab is significantly increased after TBI along with synchronous changes in BBB permeability and brain water content,indicating that AB-Ab may be associated with the increase of BBB permeability and brain edema.

关 键 词：脑损伤 血脑屏障 脑水肿 抗脑抗体 

分 类 号：R651.15[医药卫生—外科学]