慢性乙型肝炎患者外周血中T淋巴细胞亚群和CD4^+CD25^+调节性T淋巴细胞的表达水平与HBV DNA定量的相关分析  

Correlation analysis of percentages of T lymphocyte subsets and CD4^+CD25^+ regulatory T cells and HBV DNA level in peripheral blood of chronic hepatitis B patients

作　　者：李彩东[1] 陈锡莲[1] 田鹏飞[1] 李惠军[1] 吴斌[1] 

机构地区：[1]兰州市第二人民医院肝病研究所,兰州730046

出　　处：《临床肝胆病杂志》2015年第4期541-545,共5页Journal of Clinical Hepatology

基　　金：兰州市科技发展指导性计划项目(2013-ZD-06)

摘　　要：目的探讨慢性乙型肝炎(CHB)患者外周血中T淋巴细胞亚群(CD3+T、CD4+T、CD8+T、CD4+/CD8+T)和CD4+CD25+调节性T淋巴细胞(Treg)表达水平与HBV DNA定量的相关性。方法选取2012年7月-12月在兰州市第二人民医院就诊的175例HBV感染者作为研究对象,分为CHB组(63例)、HBV携带者(ASC组)(112例),以同期50例健康体检者作为对照组。采用流式细胞术检测3组患者血清中的CD3+T、CD4+T、CD8+T、CD4+T/CD8+T和CD4+CD25+Treg表达水平,实时荧光定量PCR法检测HBV DNA载量。多组间均数比较采用方差分析,进一步两两比较采用LSD-t检验,计数资料组间比较采用χ2检验;采用Pearson进行相关性分析。结果 CHB患者外周血CD4+CD25+Treg表达水平(30.97±18.78)%显著高于对照组(27.30±17.59)%,差异具有统计学意义(P<0.05);与对照组相比,CHB组、ASC组的CD3+T、CD4+T淋巴细胞百分数和CD4+与CD8+T淋巴细胞百分数比值显著降低,差异均有统计学意义(P值均<0.05),CD8+T淋巴细胞百分数显著升高,差异均有统计学意义(P值均<0.05);ASC组和CHB组CD4+CD25+Treg表达水平与HBV DNA呈正相关(r值分别为0.501、0.682,P值均<0.01)。结论 HBV感染者存在T淋巴细胞亚群比例异常,HBV DNA可以促进CD4+CD25+Treg表达水平升高,说明CD4+CD25+Treg和CD4+与CD8+T淋巴细胞比例失衡在乙型肝炎慢性化过程中发挥重要作用。Objective To investigate the correlation between the percentages of T lymphocyte subsets （CD3 ＋T,CD4 ＋T,CD8 ＋T,and CD4 ＋/CD8 ＋T）and CD4 ＋CD25 ＋regulatory T cells （Treg cells）and hepatitis B virus （HBV）DNA level in the peripheral blood of chro-nic hepatitis B （CHB）patients.Methods We chose 175 patients with HBV infection,who visited The Second People′s Hospital of Lanzhou from July to December,2012.The patients were divided into CHB group （n=63）group and asymptomatic carrier （ASC）group （n=1 12）.In the same period,50 healthy examinees served as control group.The percentages of CD3 ＋,CD4 ＋,CD8 ＋,and CD4 ＋/CD8 ＋T lymphocytes and CD4 ＋CD25 ＋Treg cells in the serum of three groups were measured by flow cytometry.HBV DNA level was ana-lyzed by quantitative real-time PCR.The comparison of means between multiple groups was performed by analysis of variance.Further comparison between any two means was performed by LSD-t test.Correlation analysis was performed by Pearson′s correlation test.Results The percentage of CD4 ＋CD25 ＋Treg （30.97%±18.78%）in the peripheral blood of CHB patients was significantly higher than that of the control group （27.30%±17.59%）（P〈0.05）.Compared with the control group,the CHB and ASC groups had significantly reduced percentages of CD3 ＋and CD4 ＋T lymphocytes and CD4 ＋/CD8 ＋T lymphocyte ratio （P〈0.05）and a significantly increased percentage of CD8 ＋T lymphocytes （P〈0.05）.There was a positive correlation between the percentage of CD4 ＋CD25 ＋Treg cells and HBV DNA level in the ASC and CHG groups （r=0.501 and 0.682,P〈0.05 for both）.Conclusion There are normal percentages of T lymphocyte sub-sets in HBV infection patients.HBV DNA can lead to an increased percentage of CD4 ＋CD25 ＋Treg cells,indicating that the imbalance of CD4 ＋CD25 ＋Treg and CD4 ＋/CD8 ＋T cells plays an important role in the chronic progression of hepatitis B.

关 键 词：肝炎 乙型 慢性 T淋巴细胞 调节 T淋巴细胞亚群 病毒载量 

分 类 号：R512.62[医药卫生—内科学]