受激拉曼散射显微技术在生物科学中的应用  

Stimulated Raman scattering microscopy and its application in biological sciences

作　　者：满奕[1] 李昂[2,3] 曹德昌 林金星[1] 黄岩谊[2,3] 荆艳萍[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京大学生物动态光学成像中心 [3]北京大学工学院,北京100871

出　　处：《电子显微学报》2015年第2期154-162,共9页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.31371348);长江学者和创新团队发展计划资助(No.IRT13047)

摘　　要：受激拉曼散射(stimulated Raman scattering,SRS)显微技术,可以通过探测分子的特定振动模式,实现对活细胞的无标记和新型非荧光标记成像。由于SRS显微术具有较高的化学特异性,在生命科学成像领域具有极大的应用潜力。本文简要介绍了SRS显微技术的原理,同时总结了其在脂类、核酸和蛋白质等生物分子,以及在组织结构、小分子药物和食品分析中的应用。Stimulated Raman scattering （ SRS ） microscopy offers a label-free live cell imaging via detecting specific vibration of chemical bonds within particular molecules. Because of its high specificity, SRS is a promising imaging method for in vivo studies. This review mainly focuses on the principle of SRS Microscopy and its application in biological sciences, especially in the imaging of biological molecules such as lipids, nucleic acids and proteins, as well as tissues, agricultural chemicals and foods.

关 键 词：受激拉曼散射显微技术 生物学 非标记成像 

分 类 号：Q-336[生物学]