孕期边缘型维生素A缺乏对新生鼠RARα、Src和NMDAR1表达的影响  

Effects of marginal vitamin A deficiency in pregnancy on expressions of retinoic acid receptor alpha, Src, and NMDAR1 of newborn rats

作　　者：陈丽君[1,2] 任兰[1] 刘祖银 李廷玉[1,3] 陈洁[1] 张萱[1,3] 

机构地区：[1]重庆医科大学附属儿童医院儿童发育疾病研究省部共建教育部重点实验室,重庆400014 [2]重庆医科大学公共卫生与管理学院,重庆400016 [3]重庆医科大学附属儿童医院儿童保健科,重庆400014

出　　处：《上海交通大学学报（医学版）》2015年第4期500-505,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金青年基金(81200483);高等学校博士学科点专项科研基金(新教师类;20125503120006)~~

摘　　要：目的探讨孕期边缘型维生素A缺乏(MVAD)对新生鼠海马中视黄酸核受体α(RARα)、肉瘤基因Src和N-甲基-D-天门冬氨酸受体1(NMDAR1)表达的影响。方法构建孕期维生素A正常(VAN)和MVAD大鼠模型(VAN组和MVAD组);分离培养新生鼠原代海马神经元,免疫荧光染色鉴定;采用real-time PCR和Western blotting检测新生鼠海马组织及体外培养的原代海马神经元中RARα、Src和NMDAR1的mRNA和蛋白表达;利用钙影像仪检测原代海马神经元钙兴奋性。结果免疫荧光染色显示,分离培养的新生鼠原代海马神经元95%表达神经元标志物神经元特异性烯醇化酶(NSE);MVAD组新生鼠海马组织和原代海马神经元中RARα、Src、NMDAR1的mRNA和蛋白表达水平明显低于VAN组(P<0.05);MVAD组新生鼠海马神经元的钙兴奋性明显低于VAN组(P<0.05)。结论孕期MVAD会降低海马RARα、Src和NMDAR1的表达,可能与孕期MVAD影响幼鼠的学习记忆功能有关。Objective To explore the marginal vitamin A deficiency（ M V AD） in pregnancy on the expressions of retinoic acid receptor alpha（ RARα）, sarcoma gene Src, and N-methyl-D-aspartate receptor subunit 1（ N M DAR1） in the hippocampus of newborn rats.Methods The pregnant rat models of vitamin A normal（ V AN group） and marginal vitamin A deficiency（ M V ADgroup） were established.The hippocampus tissue of newborn rats was isolated.The primary hippocampus neurons were cultured in vitro and detected by the immunofluorescence staining.The mRN A and protein expressions of RARα, Src, and N M DAR1 in hippocampus tissue and primary hippocampus neurons of newborn rats were detected by the real-time PCR and W estern blotting.The calciumexcitability of primary hippocampus neurons was detected by the calciumimaging instrument.Results The results of immunofluorescence staining showed that 95% of primary hippocampus neurons of newborn rats that were isolated and cultured in vitro expressed the neuron maker of neuron-specific enolase（ N SE）.The mRN A and protein expressions of RARα, Src, and N M DAR1 in hippocampus tissue and primary hippocampus neurons of newborn rats of the M V ADgroup were significantly lower than those of the V AN group（ P〈0.05）.The calciumexcitability of hippocampus neurons of newborn rats of the M V ADgroup was significantly lower than that of the V AN group（ P〈0.05）.Conclusion M V ADin pregnancy can decrease expressions of RARα, Src, and N M DAR1 in the hippocampus.This may be relevant to the impaired learning and memory functions of newborn rats caused by M V ADin pregnancy.

关 键 词：孕期 边缘型维生素A缺乏 视黄酸核受体α 肉瘤基因 N-甲基-D-天门冬氨酸受体1 

分 类 号：R714[医药卫生—妇产科学]