慢性不完全性睡眠剥夺对幼鼠脂肪细胞因子表达的影响  

Effects of chronic sleep deprivation on expression of adipocytokine of young rats

作　　者：陈喆[1] 杨倩[1] 陈婷[1] 陈炉[1] 张泽彧[1] 严国锋[2] 戴斐[3] 郑志杰[3] 李生慧[3] 

机构地区：[1]上海交通大学医学院2010级预防医学班,上海200025 [2]上海交通大学医学院实验动物科学部,上海200025 [3]上海交通大学公共卫生学院,上海200025

出　　处：《上海交通大学学报（医学版）》2015年第4期614-617,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家大学生创新性实验计划(201310248080)~~

摘　　要：目的探索慢性不完全性睡眠剥夺对幼鼠循环脂肪细胞因子表达的影响。方法 27只3周龄雄性SD大鼠,随机分为睡眠剥夺组(剥夺组)、睡眠剥夺恢复组(恢复组)、对照组。采用旋转滚轮法建立睡眠剥夺模型。采用酶联免疫吸附法检测血清瘦素、脂联素、视黄醇结合蛋白4(RBP-4)及内脏脂肪特异性丝氨酸蛋白酶抑制剂(Vaspin)的表达水平。采用李氏指数评价幼鼠体质指数。结果与对照组相比,剥夺组李氏指数较大(P<0.05);RBP-4在剥夺组和恢复组的表达水平显著降低(P<0.1);未发现瘦素、脂联素、Vaspin在各组间具有显著性差异。结论慢性睡眠剥夺可引起幼鼠肥胖程度增加、RBP-4在血清中的表达水平降低;且随着睡眠的恢复,这些影响没有得到显著改善。Objective To investigate the effects of chronic sleep deprivation on the expression of circulating adipocytokine of young rats.Methods A total of 27 three-week-old Sprague-Dawley（ SD） rats were randomly divided into the sleep deprivation group, recovery group, and control group.A motor-driven walking wheel method was adopted to establish the sleep deprivation model.The expressions of serumcytokines,including leptin, adiponectin, RBP-4, and V aspin, were detected by the ELISA.The LEE＇s index was used to evaluate the adiposity status of rats.Results The LEE＇s index of the sleep deprivation group was significantly higher than that of the control group（ P〈0.05）.Compared with the control group, RBP-4 levels of the sleep deprivation group and recovery group significantly decreased（ P〈0.1）.Expressions of leptin, adiponectin, and V aspin of 3 groups were not significantly different.Conclusion Chronic sleep deprivation results in the increase of adiposity status and decrease of RBP-4 expression in serum, which do not improve significantly with the recovery of sleep.

关 键 词：幼鼠 肥胖 慢性不完全睡眠剥夺 脂肪细胞因子 

分 类 号：R589.2[医药卫生—内分泌]