自发性2型糖尿病OLETF大鼠骨质量特点的研究  

Characteristics of bone property in spontaneous diabetic OLETF rats

作　　者：程彦臻[1] 陈珺[2] 王丹[3] 王际语 张桦[1] 杨力[1] 

机构地区：[1]南方医科大学珠江医院内分泌科,广州510282 [2]广东药学院生命科学与生物制药学院,广州510006 [3]广东省口腔医院牙周种植科,广州510280

出　　处：《中国骨质疏松杂志》2015年第4期395-398,共4页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81270966);广东省自然科学基金(S2012010009494);南方医科大学科研启动基金(PY2013N050)

摘　　要：目的观察自发2型糖尿病OLETF大鼠骨质量和骨代谢的特点。方法选用4周龄雄性糖尿病OLETF大鼠8只,雄性LETO大鼠8只作为同种系正常对照,予常规饲料喂养。实验期间每周记录各组实验动物体重和进食量,计算每周每只动物的每天平均进食量。24周龄时处死所有大鼠,检测血糖、血清胰岛素、骨代谢相关指标,取两侧股骨、两侧胫骨和L4、L5,用于Micro-CT观察骨微结构和骨形态计量学分析以及骨生物力学测定。结果与对照组相比,OLETF组大鼠体重显著增加(P<0.05),空腹血糖、胰岛素明显升高(P<0.05),骨形成指标骨钙素明显降低(P<0.05),骨吸收指标抗酒石酸酸性磷酸酶明显升高(P<0.05),血钙、血磷差异无统计学意义(P>0.05);两组大鼠骨密度的差异无统计学意义(P>0.05);Micro-CT显示OLETF组大鼠骨量下降,骨质量也明显下降,骨质受损较LETO组大鼠严重;骨生物力学数据显示,OLETF组大鼠股骨最大负荷和刚度均低于LETO组(P<0.05)。结论自发OLETF大鼠骨质改变明显,是观察2型糖尿病对哺乳动物骨质和骨量变化的较好的参考模型。Objective To investigate the characteristics of bone quality and bone metabolism in type 2 diabetic OLETF rats. Methods Eight 4-week-old male OLETF rats and 8 male LETO rats were selected.The rats in both groups were fed with routine fodder.The body weight and amount of daily intake of the rats were recorded.The rats were executed when they were 24 weeks of age.Serum glucose, insulin, and bone turnover makers were detected.Bilateral femurs, bilateral tibia, and the lumbar vertebrae L4 and L5 were collected.They were used for bone histomorphormetry analysis using micro-CT and bone biomechanical test.Results Compared with those in control group, the body weight, serum glucose, and insulin increased significantly in rats of OLETF group （P〈0.05）.The activity of serum osteocalcin in OLETF rats decreased significantly, while the activity of serum TRAP increased significantly （ P〈0.05 ） .There was no significant difference between the two groups in serum calcium, phosphorus, and bone mineral density （P〉0.05）.Micro-CT showed that bone mass and bone quality decreased more in OLETF group than in LETO group.Biomechanics results suggested that the maximal loading stiffness of the femurs were lower in OLETF group than in LETO group （P〈0.05）.Conclusion The bone change is obvious in OLETF rats, which may be a useful model to investigate bone abnormalities in obese type 2 diabetes.

关 键 词：2型糖尿病 OLETF大鼠 骨质量 

分 类 号：R681[医药卫生—骨科学]