调节性B细胞在系统性红斑狼疮患者外周血中的表达  

Expression of regulatory B cells in peripheral blood of patients with systemic lupus erythematosus

作　　者：蔡小燕[1] 李欣颖[1] 林小军[1] 唐莼[1] 叶静华[1] 李伟念[1] 何志翔[1] 李芳菲[1] 许艳丽[2] 

机构地区：[1]广州医科大学附属广州市第一人民医院风湿科,510180 [2]广州医科大学附属广州市第一人民医院中心实验室,510180

出　　处：《中华医学杂志》2015年第17期1310-1313,共4页National Medical Journal of China

基　　金：广东省科技厅科研基金项目（20128031800274）;广东省科技厅科研基金项目（20128061700006）

摘　　要：目的 分析系统性红斑狼疮（SEE）患者外周血中调节性B细胞（Bregs）的表达,探讨Bregs在SLE发病机制的作用.方法 选择60例SLE患者（其中活动期38例,稳定期22例）和20例健康对照者为研究对象,分离外周血单个核细胞（PBMC）,以CD40L及CPG寡核苷酸刺激PBMC并进行培养,用流式细胞术分析外周血IL-10＋ CD19＋ Breg和CD19＋ CD24hi CD38hi Breg的表达;ELISA方法检测培养上清中白细胞介素（IL）-10的水平.结果 （1）活动期SLE患者外周血中IL-10＋ CD19＋Breg细胞的表达为（1.54±0.64）％,稳定期SLE患者组为（2.42±0.75）％,两组比较P＜0.01;活动期和稳定期SLE组IL-10＋ CD19＋ Breg细胞的表达均低于正常对照组[（4.35±1.00）％]（P＜0.01）.（2）活动期SLE患者外周血中CD19＋ CD24hi CD38hi Breg细胞的表达为（1.26±0.45）％,稳定期SLE患者组为（2.01±0.61）％,两组比较P ＜0.01;活动期和稳定期SLE组CD19＋ CD24hiCD38hiBreg细胞的表达均低于正常对照组[（3.14±0.87）％]（P＜0.01）.（3）活动期SLE患者细胞培养上清中IL-10的水平低于稳定期SLE患者组（P＜0.05）和正常对照组（P＜0.01）;SLE患者外周血IL-10＋CD19＋ Breg和CD19＋ CD24hiCD38hiBreg细胞的表达与细胞培养上清中IL-10的水平呈正相关（r=0.652,r =0.574,均P＜0.01）.结论 活动期SLE患者外周血中IL-10＋ CD19＋ Breg和CD19＋ CD24hiCD38hiBreg显著降低,提示调节性B细胞可能参与SLE的发病.Objective To explore the expression of regulatory B cells （Bregs） in peripheral blood of patients with systemic lupus erythematosus （SLE） and elucidate the function of Bregs in the pathogenesis of SLE.Methods A total of 38 active SLE patients,22 inactive SLE subjects and 20 healthy controls were enrolled.The expressions of IL-10＋ CD19 ＋ Breg and CD19＋ CD24hi CD38hi Breg in peripheral blood mononuclear cells （PBMC） were evaluated by flow cytometry.And IL-10 was measured in the culture supermatants by enzyme-linked immunosorbent assay （ELISA）.Results The expression of IL-10 ＋ CD19 ＋ Breg in PBMCs of active SLE patients was （1.54 ± 0.64） %.And it was lower than those in inactive SLE patients （2.42±0.75）% （P〈0.01） and healthy controls （4.35 ± 1.00）% （P 〈0.01）.And the expression of CD19 ＋ CD24hiCD38hiBreg was （1.26 ±0.45）%.Also it was lower than those in inactive SLE subjects （2.01 ± 0.61） % （P 〈 0.01） and healthy controls （3.14 ± 0.87） % （P 〈 0.01）.The level of IL-10 significantly decreased in culture supermatants of active SLE patients.And it was lower than those in inactive SLE subjects （P 〈 0.05） and healthy controls （P 〈 0.01）.The percentage of IL-10 ＋ CD19 ＋ Breg and CD19 ＋ CD24hiCD38hiBreg in PBMCs of SLE patients was positively correlated with the level of IL-10 in culture supernatants （r=0.652,P〈0.01 and r =0.574,P〈0.01）.Conclusion The expression of IL-l0-related Bregs significantly decreases in PBMCs of active SLE patients.And Bregs may play some role in the pathogenesis of SLE.

关 键 词：红斑狼疮 系统性 调节性B细胞 白细胞介素-10 

分 类 号：R593.241[医药卫生—内科学]