大鼠肾性骨病模型皮质骨骨量及结构的变化  

The cortical bone mass and structure in a mouse model of renal osteodystrophy

作　　者：陈珺[1] 孟彦[2,3] 钟雁[1] 张豪[1] 杨国柱[1] 陆幸妍[1] 李敏[1] 左力[2,3] 李青南[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广州510006 [2]北京大学第一医院肾内科 [3]北京大学肾脏病研究所教育部肾脏病重点实验室教育部慢性肾脏病的预防与治疗重点实验室

出　　处：《肾脏病与透析肾移植杂志》2015年第2期113-116,共4页Chinese Journal of Nephrology,Dialysis & Transplantation

摘　　要：目的：观察腺嘌呤联合高磷饮食建立的肾性骨病模型大鼠的皮质骨骨量和骨结构的形态学变化,并探讨其骨矿代谢情况及机制. 方法：3月龄雄性SD大鼠24只,随机分为腺嘌呤模型组（M组,1～4周予含腺嘌呤的高磷饮食,5～6周高磷饮食饲养）和高磷饮食对照组（C组,高磷饮食饲养6周）;皮下注射钙黄绿素进行体内双荧光标记,处死大鼠后取左侧胫骨中段不脱钙包埋制备骨标本,骨组织形态计量学分析骨量和骨结构变化情况.结果：静态参数显示：与C组相比,M组骨组织总面积明显变小、皮质骨面积百分比和皮质骨厚度显著降低,骨髓腔面积百分比明显增大;胫骨皮质上出现侵蚀小孔.动态参数显示：与C组相比,M组骨外膜面荧光标记周长百分比无显著变化,但骨矿化沉积率、骨形成率明显增加;骨内膜面荧光标记周长百分比、骨矿化沉积率明显提高,骨形成率和骨吸收周长百分数明显增加. 结论：腺嘌呤作用下高磷饮食大鼠胫骨皮质骨的骨外膜、内膜的骨形成和吸收活跃;皮质骨内外膜骨量丢失,说明骨吸收大于骨形成、骨偶联失衡而导致皮质厚度减少,处于明显的骨高转化代谢状态.Objective：To observe the cortical bone changes in the renal osteodystrophy model by feeding an adenine and high phosphorus diet to rats, and discuss the effects of an adenine with high phosphorus diet on bone mineral metabolism. Methodology： Twenty four cases of male SD rats were randomly divided into control group （ C group were fed on high phosphorus diet for all 6 weeks ） and model group （ M group were fed an adenine and high phosphorus diet for 4 weeks followed a high phosphorus diet without adenine for 2 weeks）. All rats were injected subcutaneously with calcein （ 10 mg/kg） at 13, 12, 3, and 2 days before sacrifice for fluorescent labeling. The left tibia shaft was taken for bone histomorphometry after uncalcified embedded. Results： Bone histomorphometry static parameters showed that total tissue area, percent cortical area and cortical width were significantly decreased in M group, while the percent marrow area was increased, comparing with those in C group. Erosion holes were found in cortical bone. Dynamic parameters displayed that no significant difference was founded in percent labeled perimeter while mineral apposition rate and bone formation rate were significantly increased in M group, compared with the C group on periosteum surface. While parameters on endosteum surface, such as percent labeled perimeter, mineral apposition rate, bone formation rate and percent eroded perimeter were significantly increased. Conclusion ： The activation of bone formation and bone resorption on periosteum and endosteum surface were observed, and the reason for bone mass and thickness reduction involved the bone resorption rate is greater than bone formation rate. Cortical bone was obviously presented high bone turnover in rat feeding an adenine with high phosphorus diet. Erosion holes in cortical bone showed the destruction of bone structure.

关 键 词：肾性骨病模型 腺嘌呤 高磷饮食 骨高转换 皮质骨 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学] R68[医药卫生—临床医学]