猪同种异体原位左肺移植模型的建立  

A porcine model of orthotopic left lung transplantation

作　　者：曹浩[1] 周文勇[2] 康乐[2] 占贞贞[2] 王竹林[2] 汪波[2] 丁海燕[1] 范慧敏[1,2] 刘中民[1,2] 

机构地区：[1]同济大学附属东方医院心胸外科,上海200123 [2]同济大学附属东方医院转化医学中心,上海200120

出　　处：《中国实验动物学报》2015年第2期111-114,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：浦东新区卫生系统领先人才培养(PWR12011-01);上海市重点专科心脏外科(ZK2012A27);上海市浦东新区卫生系统重点学科群(Grant No.PWZxq2014-01);教育部高等学校博士学科点专项科研基金(20130072120062);上海市浦东新区卫生;计生科技项目(PW2013A-2);上海市青年科技启明星计划(13QA1403100);同济大学青年优秀人才培养计划(2012KJ043);上海市东方医院启航计划(DFQHM4)

摘　　要：目的建立接近于人的猪同种异体左肺原位移植动物模型。方法环江香猪12只作为供体,巴马香猪12只作为受体,左侧第4肋间开胸,完成左肺原位移植。术后1、2、4、6、12 h开胸测左、右肺动脉的压力,同时取左、右肺静脉血进行血气分析,取左、右肺组织,观察含水量及病理学改变。结果动物术后均存活,随着术后时间延长,供肺静脉血氧合指数(Pa O2/Fi O2)下降和肺动脉压(PAP)上升,与受体正常肺比较,差异有显著性(P<0.05)。随着时间的推移,移植肺组织出现水肿、炎性细胞浸润、红细胞渗出,肺泡壁增厚明显,部分肺泡腔完全闭塞,部分肺组织实变等变化,与受体肺组织比较,含水量增加显著(P<0.05)。结论为研究肺移植缺血再灌注损伤及免疫排斥反应研究机制提供了理想的动物模型。Objective To establish a porcine allogeneic left lung orthotopic transplantation model to closely simu-late human lung transplantation.Methods Twelve Huanjiang mini-pigs were used as donors and 12 Bama mini-pigs as recipients.The left lung orthotopic transplantation was completed by the left fourth intercostal thoracotomy.At 1 h, 2 h, 4 h, 6 h, 12 h after transplantation, the left and right pulmonary artery pressure were measured, the left and right pulmonary vein blood gas was analyzed, and samples of the left and right lung tissues were taken to determine the water content and for pathological examination.Results All animals survived, and the transplanted pulmonary vein blood PaO2/FiO2 and PAP were rised along with the prolonged postoperative time, compared with those of the recipient normal lung showing a signifi-cant difference （P〈0.05）.With the pass of time, there were increasing edema, inflammatory cell infiltration, RBC ooze, thickening of alveolar wall in the transplanted lung tissue, and some alveolar lumen occlusion and lung tissue consolidation. The water content of the transplanted lung tissue was increased significantly compared with that in the recipient lung tissue （ P〈0.05 ） .Conclusions The established method in this study provides an ideal animal model for research on lung transplantation ischemia-reperfusion injury and immune rejection mechanism.

关 键 词：肺移植 猪 动物模型 

分 类 号：Q95-33[生物学—动物学]