搏动型心室辅助装置大动物模型的建立  

Establishment of a sheep model of pulsatile ventricular assist device

作　　者：叶亮[1] 李铁岩[1] 曹浩[1] 张治国[1] 卢蓉[1] 丁海燕[2] 范慧敏[1] 

机构地区：[1]同济大学附属东方医院心外科,上海200123 [2]同济大学附属东方医院手术室,上海200123

出　　处：《中国实验动物学报》2015年第2期124-126,共3页Acta Laboratorium Animalis Scientia Sinica

基　　金：上海领军人才(2012-053);上海市浦东新区卫生系统领先人才(Grant No.PWR12011-01);上海市医学重点专科心脏外科(ZK2012A27);上海市科委重点项目(13411951402);上海市浦东新区卫生系统重点学科群(Grant No.PWZxq2014-01)

摘　　要：目的建立可用于搏动型心室辅助装置动物实验的大动物模型。方法选取实验动物小尾寒羊3只,麻醉后建立动静脉通路,左侧开胸建立体外循环,心脏诱颤,心尖部打孔缝合心尖插管,降主动脉缝合主动脉插管,连接DPVAD,启动驱动器,观察血泵运转情况和实验动物情况。结果血泵运转良好,血泵随驱动器正压负压驱动血液单向流动。同时动物左心室负荷减轻,动脉血压升高。结论建立搏动型心室辅助装置的大动物模型对于进行国产化心室辅助装置的研发具有重要意义。Objective To establish a large animal （ sheep） model to serve the experiments of domestic pulsatile ventricular assist device.Methods Three small-tail Han-sheep were anesthetized and the vein access and artery access were achieved.The cardiopulmonary bypass was established through left thoracotomy.Ventricular fibrillation was induced. An hole was made in the apex of left ventricle and the apex cannulation was sutured to it.The aortic cannulation was su-tured to the descending aorta.The two cannulations were connected to the domestic pulsatile ventricular assist device （ DP-VAD） and the driver was turned on.The working of DPVAD and the conditions of the animals were observed.Results The DPVAD worked well and uni-directional blood flow was driven by positive and negative pressure.The left ventricle was unloaded and the blood pressure was raised up.Conclusion The establishment of sheep model of pulsatile ventrieular as-sist device may play important role for the research and development of DPVAD in our country.

关 键 词：心室辅助装置 羊 动物模型 

分 类 号：Q95-33[生物学—动物学] R645.2[医药卫生—外科学]