急性放射性肝损伤大鼠模型的建立与评价  

Establishment and evaluation of a rat model of acute radioation-induced liver injury

作　　者：吴戈[1] 肖蕾[1] 张瑞丽[1] 艾尼瓦尔.艾木都拉 张华[1] 毛睿[1] 程纬[1] 王云莲[1] 杨颖[1] 包永星[1] 

机构地区：[1]新疆医科大学第一附属医院肿瘤中心,乌鲁木齐830054

出　　处：《中国实验动物学报》2015年第2期178-181,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：新疆医科大学第一附属医院院内科研奖励基金(2012YFY32);乌鲁木齐市感染与肿瘤重点实验室开放课题(WIT-2013-06)

摘　　要：目的探讨急性放射性肝损伤大鼠模型的建立及TGF-β1动态变化的意义。方法选用雄性SD大鼠40只,随机分为模型组(30只)和对照组(10只)。除对照组外,模型组大鼠右半肝均接受单次6 MV X线25 Gy照射,接受照射第3、5、10天,应用HE染色、透射电镜下观察大鼠右肝病理学变化,血清学检测TGF-β1、肝功能相关指标(ALT,AST,ALP),数据用SPSS 17.0统计软件进行分析。结果在照射第3、5、10天后,电镜下可观察肝脏早期病理学改变;TGF-β1的表达随时间延长而逐渐增加,模型组与对照组比较、不同时间段相比较,差异均有统计学意义(P<0.05);对照组和模型组的HE染色结果之间无明显差别;肝功能相关指标(ALT,AST,ALP)不同时间点比较无明显统计学意义(P>0.05)。结论电镜下观察肝脏病理学改变早于光镜下的变化,可用于早期放射性肝损伤的评价;TGF-β1较肝功能更加敏感地反映出照射后肝脏的损伤状态,可作为预测急性放射性肝损伤程度的血清细胞因子。Objective To explore the establishment of a rat model of acute radiation-induced liver injury and sig-nificance of the dynamic changes of TGF-β1 expression.Methods Forty healthy 6-week old male SD rats were randomly divided into model group （n=30） and control group （n=10）.The right liver of rats in the model group was given a single dose of 25 Gy 6 MV X-ray irradiation.Histopathological examination using HE staining and transmission electron microsco-py were conducted to observe the liver pathological changes in rats at 3, 5, and 10 days after irradiation, serum TGF-β1 was detected, and relevant indicators of liver function （ ALT, AST, ALP） were determined.Statistical analysis was per-formed using SPSS 17.0 software.Results At 3, 5 and 10 days after irradiation, early pathological changes in the liver cells were observed by electron microscopy, the expression of TGF-β1 was gradually increased with the time prolongation, and significant differences were found between the model group and the control group at different time points （P〈0.05）. The light microscopic observation of liver tissues did not show significant differences between the control group and model group.The liver ALT, AST, ALP at different time points did not show significant differences between the two groups （ P〉0.05）.Conclusion Electron microscopy can be used to evaluate the early changes of radiation-induced liver injury, pri-or to the alterations visible by routine light microscopy.TGF-β1 can be used to predict the degree of radiation-induced liver injury, and may be used as a sensitive serum cytokine in predicting the degree of radiation-induced acute liver injury.

关 键 词：放射性肝损伤 放射性肝纤维化 TGF-Β1 大鼠 

分 类 号：Q95-33[生物学—动物学]