KCL22/NOD-SCID小鼠慢性粒细胞白血病移植瘤模型的建立及其鉴定  

Establishment and verification of a KCL22/NOD-SCID mouse transplantation tumor model of chronic myeloid leukemia

作　　者：文良雪 刘鑫[1] 李会[1] 黄宁姝 黄峥兰[1] 冯文莉[1] 

机构地区：[1]重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016

出　　处：《中国实验动物学报》2015年第2期188-193,共6页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的研究人慢性粒细胞白血病细胞株KCL22在NOD-SCID小鼠体内致白血病的能力,为慢性粒细胞白血病血液移植瘤模型鼠的建立奠定基础。方法取对数生长期的KCL22细胞2×107个,经尾静脉注射入NOD-SCID小鼠,对照组小鼠注射无菌PBS。观察小鼠一般情况,瑞氏染色监测血象和骨髓象变化,PCR检测骨髓细胞BCR-ABL基因转录水平,HE染色观察肝、脾组织肿瘤细胞浸润情况。结果实验组小鼠于注射细胞后约4周开始出现反应力下降、精神萎靡、股骨肌肿大、后肢骨节出血点等体征,外周血白细胞从第5周逐渐增多,计数较对照组显著升高(P<0.05),血涂片可见幼稚粒细胞,肝、脾、骨髓组织切片可见白血病细胞浸润,骨髓细胞高表达BCR-ABL融合基因,未经治疗存活约70天,较对照组显著缩短(P<0.05)。结论 KCL22细胞可成功构建NODSCID小鼠慢性粒细胞白血病移植瘤模型。Objective To investigate the potential of chronic myeloid leukemia （ CML） cell line KCL22 in indu-cing leukemia in NOD-SCID mice for setting up a basis for constructing a CML mouse transplantation tumor model. Methods 2×10^7 KCL22 cells in logarithmic growth phase were injected via the tail vein into experimental NOD-SCID mice whereas PBS was injected to the mice of control group.General condition of the mice of both groups was observed.Wright staining was used to observe the changes of blood and bone marrow smears.PCR was conducted to detect the transcription level of BCR-ABL, and histology with HE staining was used to evaluate the tumor cell invasion in the liver and spleen. Results Four weeks after the injection of KCL22 cells, the mice in experimental group showed physical signs of decreased reactivity, depression, swollen hindlimb muscles and petechia on the hindlimb femur.Peripheral white blood cells （ WBC） began to increase after 5 weeks, with a significantly increased quantity compared with the control group （P〈0.05）.Imma-ture granulocytes could be seen in blood and bone marrow smears, and tumor cell infiltration was found in the liver and spleen.BCR-ABL was highly expressed in bone marrow cells.Survival time of the experimental mice without therapy was 70 days, significantly shorter than that in the control group （ ＆gt;90 days） （P〈0.05）.Conclusions A NOD-SCID mouse model of CML transplantation tumor is successfully established with leukemia KCL22 cells.

关 键 词：慢性粒细胞白血病 KCL22细胞 NOD-SCID小鼠 白血病 

分 类 号：Q95-33[生物学—动物学]