化学合成microRNA-21 inhibitors对大鼠心肌成纤维细胞活化增殖的影响  

The effect of microRNA-21 inhibitors on proliferation and activation of cardiac fibroblasts in rats

作　　者：张猛[1,2] 陶辉[1,2] 陈泽文[1,2] 张家贵[1,2] 宣海洋[1,2] 占红英[1,2] 石开虎[1,2] 

机构地区：[1]安徽医科大学第二附属医院心胸外科,合肥230601 [2]安徽医科大学心血管病研究中心,合肥230601

出　　处：《安徽医科大学学报》2015年第5期577-581,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金项目(编号:1308085MH117;1408085MH175);安徽高校省级自然科学研究项目(编号:KJ2011A175;KJ2012Z164)

摘　　要：目的探究microRNA-21 inhibitors对大鼠心肌成纤维细胞活化增殖的影响。方法应用LipofectamineTM2000Reagent向大鼠心肌成纤维细胞瞬时转染microRNA-21 inhibitors 24、48 h后,实时荧光定量聚合酶链反应(qRT-PCR)检测microRNA-21、I型胶原前胶原A1(Col1A1)和α-平滑肌肌动蛋白(α-SMA)的表达水平;Western blot法检测Col1A1和α-SMA的表达水平;MTT法检测microRNA-21 inhibitors对心肌成纤维细胞活化增殖的影响。结果转染microRNA-21 inhibitors的心肌成纤维细胞中,microRNA-21表达下调,Col1A1和α-SMA的表达下降;转染microRNA-21 inhibitors的心肌成纤维细胞增殖活性明显减弱。结论 microRNA-21 inhibitors可明显抑制心肌成纤维细胞增殖活性,提示microRNA-21是心肌成纤维细胞活化增殖的潜在靶向分子,有利于为干预和预防心肌纤维化的发生发展提供新思路。Objective To investigate the effect of microRNA-21 inhibitors on proliferation and activation of cardiac fibroblasts in rats. Methods LipofectamineTM 2000 Reagent was used to transfected cardiac fibroblasts with mi-croRNA-21 inhibitors. After 24,48 h, qRT-PCR was applied to assess the expressions of microRNA-21 and mRNA＆amp;nbsp;of Col1A1 and α-SMA. The protein expression levels of Col1A1 and α-SMA were detected by Western blot. MTT assay was used to determine the proliferation influence of the transfected cardiac fibroblasts. Results The cardiac fibroblasts transfected microRNA-21 inhibitors exhibited down-regulated microRNA-21 expression and attenuated Col1A1 and α-SMA mRNA expression. The cardiac fibroblasts proliferation activity decreased significantly after transfected microRNA-21 inhibitors. Conclusion MicroRNA-21 inhibitors can suppress the proliferation activity of cardiac fibroblasts significantly, implicating microRNA-21 as a potential target for cardiac fibroblasts activation and proliferation and pointing out that this result can provide a new idea for intervening and preventing the myocardial fibrosis occurrence and development.

关 键 词：MICRORNA-21 心肌成纤维细胞 COL1A1 Α-平滑肌 肌动蛋白 

分 类 号：R542.23[医药卫生—心血管疾病]