塞来昔布对人成骨细胞增殖的影响及其作用机制  

Study on the impact of celecoxib on proliferation of human osteoblasts

作　　者：高杰[1] 桂斌捷[1] 胡孔足[1] 王斯晟[1] 

机构地区：[1]安徽医科大学第一附属医院骨科,合肥230000

出　　处：《安徽医科大学学报》2015年第5期608-611,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1408085MH182)

摘　　要：目的观察塞来昔布对人成骨细胞增殖的影响及其作用机制。方法将人成骨细胞分为3个组:空白对照组、IL-1刺激组(阴性对照组)、塞来昔布+IL-1刺激组(药物干预组),用RT-PCR法和Western blot法分别检测三组成骨细胞环氧化酶-2(COX-2)、膜相关前列腺素E2合成酶1(m PGES-1)mRNA和蛋白的表达。MTT法检测塞来昔布对阴性对照组人成骨细胞增殖的影响。结果 RT-PCR法和Western blot法实验结果显示,同空白对照组相比,阴性对照组的人成骨细胞中COX-2、m PGES-1 mRNA和蛋白的表达均增加;同阴性对照组相比,药物干预组的COX-2、m PGES-1mRNA和蛋白的表达均降低。塞来昔布对IL-1刺激的人成骨细胞的生长具有抑制作用,呈现剂量依赖性,浓度越大,抑制作用越大;并且随着作用时间的延长,细胞的抑制作用增大。结论塞来昔布可以抑制炎性状态下人成骨细胞的生长,可能是通过调控COX-2、m PGES-1 mRNA和蛋白的表达,使其表达下调而发挥作用。Objective To study the impact of celecoxib on proliferation of human osteoblasts. Methods The hu-man osteoblasts were cultured and divided into three groups,including the negative control, positive control （stimu-lated by IL-1） and intervention group （celecoxib ＋ IL-1）. The detection of mRNA and protein level of COX-2 and mPGES-1 were measured by real tmie PCR （RT-PCR） and Western blot. MTT assay was performed to determine the impact of celecoxib on proliferation of human osteoblasts which were treated with IL-1. Results RT-PCR and Western blot showed that the detection of mRNA and protein level of COX-2 and mPGES-1 of the positive control （stimulated by IL-1） were significantly higher than that of the negative control and intervention group （celecoxib ＋IL-1）. Celecoxib had cytotoxic effect on the growth of osteoblasts, manifests dose dependence as well as increasing cell inhibition over time. Conclusion Celecoxib could inhibit proliferation of osteoblasts, and could inhibit the oc-currence of heterotopic ossification by regulating the level of mPGES-1 and COX-2 mRNA and protein.

关 键 词：环氧化酶-2 塞来昔布 成骨细胞 异位骨化 

分 类 号：R681.8[医药卫生—骨科学]