儿童狼疮性肾炎患者中EB病毒潜伏膜蛋白1的表达  

The expression of LMP-1 gene in juvenile lupus nephritis

作　　者：丁艳[1,2] 廖旺[1] 向伟[2] 杨慧兰[3] 何小解[4] 党西强[4] 易著文[4] 

机构地区：[1]海南省人民医院皮肤科,海口570102 [2]海南省妇幼保健院皮肤科,海口570206 [3]广州军区广州总医院皮肤科,广州510010 [4]中南大学湘雅二医院儿童医学中心小儿肾脏专科,长沙410011

出　　处：《安徽医科大学学报》2015年第5期676-679,共4页Acta Universitatis Medicinalis Anhui

基　　金：海南省自然科学基金(编号:814314;309079)

摘　　要：目的通过实时定量逆转录PCR(RT-PCR)、肾脏免疫组化和ELISA 3种方法研究儿童狼疮性肾炎(LN)中EB病毒(EBV)潜伏膜蛋白1(LMP-1)的表达,试图探讨LMP-1在儿童LN中的致病机制。方法 41例LN患儿和12例健康对照组,分别用RT-PCR法、免疫组化法、ELISA法检测两组外周血单核细胞(PBMCs)、肾脏组织及血清中LMP-1的表达。结果 1 RT-PCR法、免疫组化法、ELISA法检测LN患儿和健康对照者的阳性率分别为65.9%vs 8.3%,95.1%vs 16.7%,22.0%vs 8.3%,差异有统计学意义(P<0.05);2 3种方法检测LMP-1的表达,免疫组化法检测阳性率高于其他两种方法,差异有统计学意义(P<0.05)。结论儿童LN中存在EBV潜伏期基因LMP-1表达异常,其中肾脏组织中表达阳性率最高,提示LMP-1可能参与了儿童LN的发病。Objective To discuss the role of latent membrane protein 1 （LMP-1）in the pathgenesis of juvenile lu-pus nephritis （ LN） through investigating expression of LMP-1 by RT-PCR, immunohistochemistry and ELISA. Methods RT-PCR,immunohistochemistry and ELISA were applied to detect expression of LMP-1 in PBMCs, kid-ney tissue and serum from 20 patients with LN and 12 healthy control subjects respectively. Results ① There was signifcant difference between juvenile LN patients and healthy controls by RT-PCR, immunohistochemistry and ELISA,Positive rate were 65. 9% vs 8. 3% ,95. 1% vs 16. 7% ,22. 0% vs 8. 3% , respectively（P 〈 0. 05）. ② Pos-itive rates of LMP-1 in juvenile LN were significantly different among three methods and the positive rate detected by immunohistochemistry was higher than the other two methods （P 〈 0. 05）. Conclusion The results demonstrate that there are aberrant expression of LMP-1 gene in juvenile LN, thepositive rate of LMP-1 in kidney tissue is high-est, suggesting that LMP-1 gene may contribute to the pathgenesis of juvenile LN.

关 键 词：儿童 狼疮性肾炎 潜伏膜蛋白1 EB病毒 

分 类 号：R373.9[医药卫生—病原生物学] R758.69[医药卫生—基础医学]