Treg在慢性阻塞性肺疾病患者外周血及肺组织中的表达  

The expression of Treg in peripheral blood and lung tissue of COPD

作　　者：章颖妹 严青[1] 刘辉[1] 石建邦[1] 夏淮玲[1] 徐晓玲[1] 

机构地区：[1]安徽医科大学附属省立医院呼吸内科,合肥23000

出　　处：《安徽医科大学学报》2015年第5期679-682,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1208085MH158)

摘　　要：目的观察慢性阻塞性肺疾病(COPD)患者外周血及肺组织中调节性T淋巴细胞(Treg)的表达并与肺功能正常者进行对照比较,探讨Treg在COPD患者气道炎症中的作用以及吸烟对其表达的影响。方法收集24例肺功能正常的不吸烟者(NS组)、22例肺功能正常的吸烟者(S组)、25例患有COPD的吸烟者(COPD组)为研究对象,分别采用流式细胞术、免疫组织化学染色法检测3组研究对象外周血及肺组织中Treg表达。结果 COPD组的外周血中Treg表达均显著高于S组及NS组,差异均有统计学意义(P<0.01),S组的外周血Treg表达高于NS组,差异有统计学意义(P<0.01);COPD组的肺组织中Treg表达均显著低于S组及NS组,差异均有统计学意义(P<0.01),S组的肺组织中Treg表达水平低于NS组,差异无统计学意义;3组外周血Treg总表达与第1秒用力呼气容积(FEV1)/预计值(%)负相关(P<0.000 1),与吸烟量(年支)呈显著正相关(P<0.000 1),3组肺组织中Treg总表达与FEV1/预计值(%)无相关性(P=0.338),与吸烟量(年支)呈显著负相关(P=0.031)。结论 COPD患者外周血中Treg表达上调,其肺组织中Treg表达下调。吸烟量与外周血中Treg表达呈正相关,与肺组织中Treg表达呈负相关。Objective To observe the expression of regulatory T lymphocytes （Treg） in peripheral blood and lung tissue of patients with chronic obstructive pulmonary disease（COPD） and investigate the role of Treg in the airway inflammation of COPD and how smoking impacts the expression of Treg compared with population of normal lung function. Methods A total of 24 subjects were nonsmokers with normal lung function （NS group）, 22 subjects were smokers with normal lung function （S group） and 25 subjects were smokers with COPD （COPD group）. The percentage of Treg in peripheral blood was measured by flow cytometry, and the immune-histochemical methods were used to evaluate Treg expression in lung tissue. Results The percentage of Treg in peripheral blood signifi-cantly increased in COPD group compared with S group and NS group, the difference was statistically significant（P〈 0. 01）; the percentage of Treg in peripheral blood significantly increased in S group compared with NS group, the difference was statistically significant（P 〈 0. 01）; the Treg levels in lung tissue were significantly decreased in COPD group between S group and NS group, the difference was statistically significant（P 〈 0. 01）. The Treg levels in lung tissue were significantly decreased in S group between NS group, the difference was not statistically signifi-cant. The total percentage of Treg in peripheral blood with FEV1% pred was a negative correlation （P 〈 0. 000 1）, and there was a positive correlation with smoking index （P 〈 0. 000 1）; there was no significant correlation between Treg levels in total of lung tissue with FEV1% pred （P = 0. 338）, but with smoking index there was a negative cor-relation （P = 0. 031）. Conclusion In COPD patients, the percentage of Treg in peripheral blood is up-regulated, but Treg levels in lung tissue are down-regulated. There is a positive correlation between smoking index and the percentage of Treg, but it is negatively correlated with Treg levels in lung tissue.

关 键 词：T 淋巴细胞 调节性 慢性阻塞性肺疾病 吸烟 气道炎症 

分 类 号：R563.3[医药卫生—呼吸系统]