检测GPC3和GP73两种方法的比较及与临床的相关性  

Comparing two detection methods of GPC3 and GP73 and the correlation with clinic

作　　者：马洁[1] 汪顺才[2] 朱梦琪[3] 马久明[2] 周华[2] 虞腊青 杨永峰[4] 经继生 

机构地区：[1]江苏大学医学院临床生化检验系,镇江212013 [2]江苏省句容市人民医院感染病科,句容212400 [3]复旦大学附属华山医院传染科肝病研究室,上海200040 [4]南京市第二医院肝内科,南京156500

出　　处：《安徽医科大学学报》2015年第5期707-710,共4页Acta Universitatis Medicinalis Anhui

基　　金：江苏省卫生厅自然科学基金项目(编号:Y201311);江苏大学临床科技发展基金项目(编号:JLY20120103)

摘　　要：采用ELISA法检测70例标本(肝癌组39例、肝硬化组31例)血清中磷脂酰肌醇蛋白聚糖3(GPC3)和高尔基体蛋白73(GP73)的水平,qRT-PCR法检测相同标本外周血单个核细胞(PBMC)中GPC3 mRNA和GP73 mRNA的相对表达量。结果显示肝癌组血清中GPC3和GP73表达量显著高于肝硬化组(P<0.0 001);两组间PBMC中GPC3 mRNA和GP73 mRNA水平差异无统计学意义;相关性分析结果显示,肝硬化组和肝癌组血清中GPC3、GP73含量均有正相关性(P<0.01)。血清中GPC3和GP73的水平均能预测原发性肝癌,且GPC3和GP73的水平具有一定的相关性;PBMC中GPC3 mRNA和GP73 mRNA的表达水平不能很好的区分肝癌组与肝硬化组,提示ELISA法检测血清中GPC3和GP73的表达水平是临床预测原发性肝癌的首选方法。ELISA and qRT-PCR were used to detect the level of glypican3 （GPC3） and Golgi protein 73 （GP73） of 70 patients [primary hepatocellular carcinoma（HCC） group 39 patients and cirrhosis group 31 patients] in ser-um and peripheral blood mononuclear（PBMC） respectively. The results showed that the expression levels of GPC3 and GP73 in serum had significant difference between two groups（P 〈 0. 001）, but the relative expression of GPC3 and GP73 mRNA in PBMC had no great difference. Correlation analysis showed that the expression levels of GPC3 and GP73 in serum were positively correlated （P 〈 0. 01）. In conclusion, the expression levels of GPC3 and GP73 in serum can predict HCC, and the levels of GPC3 and GP73 have some relevance. GPC3 and GP73 mRNA levels in PBMC can′t distinguish between two groups, suggesting ELISA is the preferred method of clinical to detect the expression levels of GPC3 and GP3 in serum of HCC.

关 键 词：原发性肝癌 高尔基体蛋白73 磷脂酰肌醇蛋白聚糖3 ELISA法 qRT—PCR法 

分 类 号：R512[医药卫生—内科学]