用细胞学方法评价10种抗生素的寒热药性  

Evaluation of the hot and cold characteristics of ten antibiotics by cytological method

作　　者：王思文[1] 刘建利[1] 

机构地区：[1]西部资源生物与现代生物技术教育部重点实验室,西北大学生命科学学院,陕西西安710069

出　　处：《西安交通大学学报（医学版）》2015年第3期408-413,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助(No.20872118;No.30070905);陕西省重大科技专项资助(No.2008ZDKG-67)~~

摘　　要：目的评价10种常用抗生素的寒热药性。方法用噻唑蓝(MTT)比色法检测10种常用抗生素对SMMC7721以及MCF-7细胞体外增殖的影响,并用倒置显微镜观察其对细胞形态特征的影响。结果氨苄西林、头孢克肟、头孢泊肟酯、头孢克洛、头孢氨苄、阿奇霉素、克拉霉素、罗红霉素、多西环素、土霉素共10种抗生素均表现为寒性。形态学观察也表明这些药物可使细胞密度减小,固缩变圆。结论细胞学方法能够用于西药寒热药性的评价,这为西药中药化提供了一种简单实用的评价寒热药性的方法,必将促进西药中药化研究的进程。Objective To evaluate the hot and cold characteristics of 10 antibiotics.Methods MTT assay was used to investigate the effects of 10 antibiotics on the growth and proliferation of cultured SMMC7721 cells and MFC-7 cells in vitro .Morphological changes were observed under the inverted microscope.Results The 10 antibiotics,namely,ampicillin,cefixime,cefpodoxime proxetil,cefaclor,cefalexin,azithromycin,clarithromycin, roxithromycin,doxycycline and oxytetracycline showed cold or cool characteristics.Morphological observation showed that cells treated with these drugs presented decreased cell density and turned round.Conclusion The results of this study demonstrated that the cytological method can be used to evaluate the hot and cold characteristics of western drugs.This simple and reliable method will promote research on Chinese medicalization of western drugs.

关 键 词：西药中药化 抗生素 MTT法 SMMC7721细胞 MCF-7细胞 寒热药性 

分 类 号：R28[医药卫生—中药学]