大鼠激素性股骨头坏死模型的建立和评价  

Establishment and Assessment of Rat Models of Glucocorticoid-induced Osteonecrosis

作　　者：董玉雷[1] 周磊[1] 李玉龙[1] 肖刻 翁习生[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院骨科,北京100730

出　　处：《中国医学科学院学报》2015年第2期152-156,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(81272009)~~

摘　　要：目的建立大鼠激素性股骨头坏死动物模型,并进行影像学和病理评价。方法将30只成年雄性SD大鼠随机分为两组:实验组给予腹腔注射两次脂多糖(20μg/kg),每次间隔24 h,24 h后肌肉注射大剂量甲强龙(40 mg/kg),连续注射3次,每次间隔24 h,对照组给予等量生理盐水。4周后两组各随机处死5只大鼠。8周后处死剩余全部大鼠。大鼠双侧股骨标本行微计算机断层扫描,观察骨小梁微观结构变化,计算骨小梁参数。股骨头脱钙处理后行HE染色组织病理学检查。结果根据病理切片结果,实验组有12只出现骨坏死表现,建模成功率80%。4周时实验组病理切片出现空骨陷窝,8周时HE染色可观察到空骨陷窝、骨小梁断裂、纤维结构增生。8周时实验组微计算机断层扫描显示骨小梁结构紊乱、断裂、囊变,骨小梁定量分析显示骨小梁相对体积(实验组:0.55±0.13,对照组:0.68±0.12;P<0.05)、相对面积(实验组:20.45±5.13,对照组:24.00±5.20;P<0.05)、骨小梁数量(实验组:5.67±0.50,对照组:6.24±0.96;P<0.05)等指标明显低于对照组。结论脂多糖联合大剂量甲强龙可成功建立大鼠激素性股骨头坏死动物模型,使用高分辨微计算机断层扫描可进行骨小梁形态和结构的定性定量分析,与HE染色病理切片具有较好的一致性。Objective To establish the rat models of glucocorticoid-induced osteonecrosis and evaluate the osteonecrosis by high-resolution micro-CT and histomorphology. Methods Totally 30 Sprague-Dawley male adult rats（ age： 12 weeks; body weight： 400-450 g） were randomized into two groups： one group received two doses of intraperitoneal injection of lipopolysaccharide（ 20 μg / kg） at an interval of 24 hours,24 hours later,the rats received three doses of intramuscular injection of methylprednisolone（ 40 mg / kg） at an interval of 24hours; in the control group,the rats received same amount of normal saline. Four weeks later,5 rats in each group were sacrificed randomly. Eight weeks later,all the remaining rats were sacrificed and the femur specimens were scanned by micro-CT. The trabeculae parameters of the femoral head were calculated. The hematoxylin and eosin staining were performed to calculated the success rate. Results The incidence of osteonecrosis was 80% in the experimental group. The micro-CT demonstrated broken and cystic degeneration. The quantitative analysis showed that the bone volume / total volume（ experimental group： 0. 55 ± 0. 13 vs. control group： 0. 68 ± 0. 12;P 〈0. 05）,bone surface area / bone volume（ experimental group： 20. 45 ± 5. 13 vs. control group： 24. 00 ± 5. 20;P 〈0. 05）,and trabeculae number（ experimental group： 5. 67 ± 0. 50 vs. control group： 6. 24 ± 0. 96; P 〈0. 05） were significantly lower in experimental group than those in control group. Conclusions A rat model of glucocorticoid-induced osteonecrosis was successfully established by lipopolysaccharide and methylprednisolone. High-resolution micro-CT is useful for the qualitative and quantitative analyses of the morphology and structure of trabeculae,showing good consistence with the histomorphological findings.

关 键 词：糖皮质激素 股骨头坏死 微计算机断层扫描 

分 类 号：R681.8[医药卫生—骨科学]