NADPH氧化酶参与AtTOR调控盐胁迫诱导自噬机制的研究  

Mechanism Study of NADPH Oxidase Involved in Regulation of Salt Stress-induced Autophagy through At TOR in Arabidopsis thaliana

作　　者：刘姿娴[1] 刘建[1] 王永强[1] 曾礼漳[1] 邢达[1] 

机构地区：[1]华南师范大学生物光子学研究院激光生命科学研究所暨激光生命科学教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2015年第2期152-157,共6页Acta Laser Biology Sinica

基　　金：国家高技术研究发展计划(863计划)课题(2007AA10Z204);教育部"长江学者与创新团队计划"创新团队项目(IRT0829);国家自然科学基金-广东联合基金重点项目(U0931005)

摘　　要：盐胁迫严重限制着植物的生长发育,造成农业产量下降。植物遭受盐胁迫时,细胞代谢受到抑制,体内会积累较多活性氧(ROS)进而对植物造成氧化胁迫,诱导自噬现象的产生。本文主要研究了植物对于盐胁迫诱导的自噬及其反应的调控机制。研究中发现,在高盐浓度处理的拟南芥幼苗中自噬现象迅速产生,伴随着NADPH氧化酶活性的明显上升。此外,通过用荧光探针Lyso Tracker Red(LTR)定位自噬小体,激光共聚焦观察发现At TOR不仅可以在正常生理环境下抑制自噬小体的生成,而且可以在高盐浓度胁迫环境下抑制自噬。进而我们发现在高盐浓度处理的同时添加NADPH氧化酶抑制剂DPI后,处理过后的WT株系拟南芥根细胞自噬现象受到明显抑制,而At TOR突变体中并未有明显的变化。因此NADPH氧化酶很有可能参与At TOR对盐胁迫诱导自噬的信号通路的调控。该研究结果为进一步分析植物耐受性机理和自噬的信号通路提供理论依据。Salinity seriously limits plant growth and development,reducing the crop productivity.Downregulation of cellular metabolism during salt stress causes reactive oxygen species （ROS）accumulation,contributing to oxidative stress and thus inducing autophagy.In this article we put emphasis on specific autophagic activity in plants during salt stress and its regulation mechanism.We found that autophagy was induced in the short term when treated with high salt concentrations,which was accompanied by a large increase in NADPH oxidase activity.In addition,we used LysoTrack-er Red （LTR）fluorescent probe as an indicator of autophagy to find that AtTOR inhibits the formation of autophagic body both in normal conditions and high salt stress conditions.After added with NADPH inhibitor DPI,autophagy was found significantly inhibited in WT during treatment with high concentrations of salt,while,no remarkable change was found in AtTOR mutant.Therefore NADPH oxidase is likely involved in the upstream regulation of AtTOR during salt stress-induced autophagy.The data may provide theoretic foundation for further studying the adaptive mechanisms and autophagic signaling pathway in plant.

关 键 词：拟南芥 自噬 盐胁迫 AtTOR NADPH氧化酶 

分 类 号：Q631[生物学—生物物理学]