绿宝石梨组织快繁技术的研究  

Study on the Rapid Propagation of Lvbaoshi Pear

作　　者：孙文英[1] 申顺先[1] 乔宝营[1] 薛丽丰[1] 卢伟娜[1] 

机构地区：[1]河南农业职业学院园艺园林系,河南郑州451450

出　　处：《中国果菜》2015年第4期49-51,共3页China Fruit & Vegetable

基　　金：河南省教育厅自然科学研究计划项目(2010C210001);河南省高等学校青年骨干教师资助计划项目(2013GGJS-252)

摘　　要：本研究以优良品种绿宝石梨的嫩茎稍为试材,对影响组织培养的主要因素,包括培养基种类、生长调节剂浓度配比进行了研究,建立了绿宝石梨的组织快繁技术。试验结果表明,适宜绿宝石梨组织培养的启动培养基由MS、1.0mg/L 6-BA、0.1mg/L IBA组成;增殖培养基是由1/2MS、1.0mg/L 6-BA、0.1mg/L IBA组成。In this paper, tender stems of Lvbaoshi pear were used as test material. Effects of media types, 6-BA and IBA with different concentrations on Lvbaoshi pear issue culture were studied, which established the fast-breeding technique of Lvbaoshi pear. The results showed that the suitable culture medium for different stages were initial medium and multiplication medium. Initial medium included MS, 1.0mg/L 6-BA, and 0.1mg/L IBA. Multiplication medium included 1/2MS, 1.0mg/L 6-BA, and IBA 0.1mg/L.

关 键 词：绿宝石梨 嫩茎 组织培养 

分 类 号：S661.2[农业科学—果树学]