离子色谱法检测饮用水中的草甘膦  

Determination of glyphosate in drinking water by ion chromatography

作　　者：李荔[1] 张巍[1] 徐建华[1] 吴嘉志[1] 

机构地区：[1]广州市白云区疾病预防控制中心,广东广州510445

出　　处：《中国卫生检验杂志》2015年第8期1152-1155,1158,共5页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立离子色谱检测饮用水中草甘膦的方法。方法以IonPacAS19(4 mm)为分析柱,EGC在线产生KOH为淋洗液,抑制电导进行检测,用草甘膦的峰面积进行定量。结果草甘膦的检出限(S/N=3)是0.015 mg/L,线性相关系数是0.999 9(0.05 mg/L^2.0 mg/L);样品加标回收率是97.7%(RSD:0.016%;0.35 mg/L^1.05 mg/L);浓度为0.1 mg/L及0.5 mg/L草甘膦样品溶液分别存放在玻璃瓶和聚丙烯塑料瓶中,连续10 d检测峰面积及保留时间,差异均无统计学意义(P>0.05);F^-等13种阴离子对饮用水中草甘膦的检测无干扰;阳离子Ca^(2+)和Cu^(2+)对其检测无影响;高浓度Cl^-对其检测有一定影响。结论该方法具有灵敏度高,操作简单,重现性好,能够快速准确检测饮用水中草甘膦的浓度。Objective To establish a method for the determination of glyphosate in drinking water by ion chromatography. Methods IonPac AS19 （4 mm） was used as analytical column, EGC online KOH as eluent, with suppressed conductivity de- tection, glyphosate was quantified by peak area. Results The detection limit of the method of glyphosate （ S/N = 3 ） was 0.015 mg/L, the linear correlation coefficient （0.05 mg/L -2.0 mg/L） was 0.999 9. The rate of recovery was 97.7% （RSD： 0. 016% ; 0.35 mg/L- 1.05 mg/L）. Samples had excellent stability, glyphosate samples with concentration of 0.1 mg/L and 0.5 mg/L were respectively stored in glass bottles and plastic bottles for 10 consecutive days＇ peak area detection and retention time, the differences were not statistically significant（ P 〉 0.05 ）, and there was no difference among the detection results ; F- and other 13 kinds of anion had no interference to the detection, Ca^2＋, Cu^2＋ had no effect on the determination, and high con- centration of Cl^- had certain influence on its determination. Conclusion Ion chromatography methods can accurately and quickly determine the concentration of glyphosate in drinking water with the advantages of high sensitivity, easy operation and good repeatability.

关 键 词：离子色谱 草甘膦 饮用水 

分 类 号：O657.75[理学—分析化学]