臭氧对腰椎间盘突出症患者髓核NO和IL-6水平的影响：离体实验  

Effects of ozone on expression of NO and IL-6 in nucleus pulposus of patients with lumbar intervertebral disc herniation in vitro

作　　者：赵菲[1] 赵学军[1] 林小雯[1] 王珺楠[1] 罗剑刚[2] 苗贵申 姜鹏[1] 傅志俭[1] 

机构地区：[1]山东大学附属山东省立医院疼痛科,济南市250021 [2]中南大学湘雅医院麻醉科

出　　处：《中华麻醉学杂志》2015年第2期178-180,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81271346）;山东省自然科学基金（ZR2010HM097）

摘　　要：目的 评价臭氧（O3）对腰椎间盘突出症患者髓核NO和IL-6水平的影响.方法 经椎间孔镜取出16例腰椎间盘突出症患者的髓核,每个髓核均分为3份,采用随机数字表法,将其分为3组（n=16）：对照组（C组）不施加任何干预;O320μg/ml组（O320组）和O340μg/ml组（O340组）分别注入20 μg/ml O3和40 μg/ml O3各2 ml.20 min后体外培养72 h,收集培养液,采用硝酸酶还原法检测NO浓度,采用ELISA法检测IL-6浓度;采用实时荧光定量PCR法检测诱导型一氧化氮合酶（iNOS） mRNA和IL-6 mRNA的表达水平.结果 与C组比较,O320组和O340组髓核培养液NO和IL-6的浓度降低,髓核组织iNOS mRNA和IL-6 mRNA的表达下调（P＜0.05）;O320组和O340组间上述指标比较差异无统计学意义（P＞ 0.05）.结论 O3治疗腰椎间盘突出症患者腰腿痛的机制与其减少椎间盘NO合成和降低局部炎性反应有关.Objective To evaluate the effects of ozone （O3） on the expression of nitric oxide （NO） and interleukin-6 （IL-6） in the nucleus pulposus of patients with lumbar intervertebral discherniation in vitro.Methods Nucleus pulposus specimens were obtained from 16 patients with lumbar intervertebral disc herniation under intervertebral foramen endoscope.Each specimen was randomly divided into 3 groups （n=16 each） using a random number table：control group （group C）,O320 μg/ml group （O320 group） and O340 μg/ml group （O340 group）.O320 and 40 μg/ml 2 ml were injected into the nucleus pulposus in O320 and O340 groups,respectively.The nucleus pulposus was cultured for 72 h in vitro 20 min later.The culture medium was collected for detection of NO and IL-6 concentrations.The nucleus pulposus tissues were obtained for determination of the expression of inducible nitric oxide synthase （iNOS） and IL-6 mRNA using real-time fluorescent quantitative PCR.Results Compared with group C,the concentrations of NO and IL-6 in the culture medium were significantly decreased,and the expression of iNOS and IL-6 mRNA in nucleus pulposus tissues was down-regulated in O320 and O340 groups.There were no significant differences in the parameters mentioned above between O320 group and O340 group.Conclusion The mechanism by which O3 treats pain in waist and legs is related to reduced production of NO in the intervetebral disc and decreased local inflammatory responses in the patients with lumbar intervertebral disc herniation.

关 键 词：臭氧 一氧化氮 白细胞介素6 椎间盘移位 

分 类 号：R681.53[医药卫生—骨科学]