mAb2G4-ODN-lip对心肌细胞缺氧复氧损伤的影响  

Effect of mAb2G4-ODN-lip on hypoxia-reoxygenation injury in H9c2 cardiomyocytes

作　　者：靖国庆 陈刘芳[1] 任瑶瑶[1] 张宗泽[1] 王焱林[1] 吴云[1] 

机构地区：[1]武汉大学中南医院麻醉科,430071

出　　处：《中华麻醉学杂志》2015年第2期211-213,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81201499）

摘　　要：目的 评价抗肌球蛋白单克隆抗体-NF-κB诱骗性寡核苷酸-阳离子脂质体复合物（mAb2G4-ODN-lip）对心肌细胞缺氧复氧损伤的影响.方法 培养H9c2心肌细胞,接种于6孔板（细胞密度1＆#215;105/ml,2 ml/孔）中,采用随机数字表法分为3组（n=9）：正常对照组（C组）细胞常规培养;单纯缺氧复氧组（H/R组）细胞进行缺氧2h,复氧1 h;mAb2G4-ODN-lip组（MOL组）缺氧前经mAb2G4-ODN-lip（2μg ODN）处理4h,更换普通培养基继续培养8h,余处理同H/R组.复氧结束时,采用甲基噻唑蓝比色法检测细胞增殖抑制率,2,4-二硝基苯肼显色法检测培养液乳酸脱氢酶（LDH）活性,硫代巴比妥酸比色法检测细胞丙二醛（MDA）含量,ELISA法检测培养液TNF-α和IL-6的浓度.结果 与C组比较,H/R组和MOL组细胞增殖抑制率、LDH活性、MDA含量、TNF-α和IL-6浓度升高（P＜0.05）;与H/R组比较,MOL组细胞增殖抑制率、LDH活性、MDA含量、TNF-α和IL-6浓度降低（P＜0.05）.结论 mAb2G4-ODN-lip（2μg ODN）可有效减轻心肌细胞缺氧复氧损伤.Objective To evaluate the influence of preconditioning with and anti-myosinmonoclonal antibody （mAb2G4）-nuclear factor-kappa B decoy oligodeoxynucleotide （ODN）-lipofectamine （lip） on hypoxia-reoxygenation （H/R） injury in H9c2 cardiomyocytes.Methods H9c2 cardiomyocytes were seeded in 6-well plate at the density of 1＆#215;105/ml （2 ml/well）,and were divided into 3 groups （n=9 each） using a random number table：control group （group C）,H/R group and mAb2G4-ODN-lip group （group MOL）.The cells underwent 2 h of hypoxia in an air-tight bag,followed by 1 h reoxygenation.In MOL group,the cells were treated with mAb2G4-ODN-lip （2 μg ODN） for 4 h and then cultured in the common culture medium for 8 h before hypoxia.At the end of reoxygenation,proliferation of cells was measured using MTT assay,and the cells and supernatant of the culture medium were collected to determine the activity of lactate dehydrogenas （LDH）,content of malondialdehyde （MDA）,concentrations of tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） （by ELISA）.The rate of proliferation inhibition was calculated.Results Compared with group C,the rate of proliferation inhibition,LDH activity,MDA content,and concentrations of TNF-α and IL-6 were significantly increased in the other two groups.Compared with group H/R,the rate of proliferation inhibition,LDH activity,MDA content,and concentrations of TNF-α and IL-6 were significantly decreased in MOL group.Conclusion mAb2G4-ODN-lip can mitigate H/R injury in H9c2 cardiomyocytes.

关 键 词：NF-κB 诱骗寡核苷酸 阳离子脂质体 肌细胞 心脏 细胞低氧 氧 

分 类 号：R542.2[医药卫生—心血管疾病]