IL-1对大鼠胸主动脉收缩功能的影响  

Effect of interleukin-1 on contractile function of rat thoracic aorta

作　　者：曹忠平 刘文娜[2] 杜洪印[3] 

机构地区：[1]天津平津医院麻醉科,300162 [2]天津医科大学研究生院 [3]天津市第一中心医院麻醉科

出　　处：《中华麻醉学杂志》2015年第2期214-217,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金面上项目（81270560）

摘　　要：目的 探讨白细胞介素1（IL-1）对大鼠胸主动脉收缩功能的影响.方法 雄性Wistar大鼠40只,体重250～ 300 g,用以制备离体胸主动脉环.实验Ⅰ 取大鼠胸主动脉环,分为2段,采用随机配伍原则分为2组（n=20）：对照组（C组）和IL-1组.IL-1组用含20 ng/ml IL-1的Kreb培养液孵育2h,再以10-9、10-8、10-7、10-6和10-5 mol/L累积浓度的苯肾上腺素（PE）诱发胸主动脉血管环收缩,C组用不含IL-1的空白培养液孵育2h,其余操作同IL-1组.实验Ⅱ 取大鼠胸主动脉环,分为3段,采用随机配伍原则分为3组（n=20）：IL-1组、IL-1＋一氧化氮合酶抑制剂L-NAME组（IL-1＋L-NAME组）和IL-1＋环氧化酶抑制剂吲哚美辛组（IL-1＋I组）.3组用含20 ng/ml IL-1的Kreb培养液孵育1.5 h,IL-1＋L-NAME组和IL-1＋Ⅰ组再分别用含100 μmol/L-L-NAME或2.5 mmol/L吲哚美辛的Kreb培养液孵育30 min,然后以10-9、10-8、10-7、10-6、10-5 mol/L累积浓度的PE诱发血管环收缩,方法同实验Ⅰ,IL-1组用不含L-NAME或吲哚美辛的Kreb培养液孵育.记录PE各浓度下胸主动脉环最大收缩张力,取与C组10-6mol/L浓度下最大收缩张力的百分比.结果 实验Ⅰ 与C组比较,IL-1组胸主动脉环对10-8、10-7、10-6和10-5mol/L PE诱发的收缩张力百分比降低（P＜0.05）.实验Ⅱ 与IL-1组比较,IL-1＋L-NAME组和IL-1＋Ⅰ组胸主动脉环对10-7、10-6和10-5 mol/L PE诱发的收缩张力百分比升高（P＜0.05）.结论 IL-1可抑制大鼠胸主动脉收缩,其机制可能与促进一氧化氮和前列环素合成有关.Objective To investigate the effect of interleukin-1 （IL-1） on contractile function of rat thoracic aorta.Methods Forty male Sprague-Dawley rats,weighing 250-300 g,were sacrificed to obtain the thoracic aortic rings.The experiment was performed in 2 parts.Part Ⅰ The thoracic aortic rings were divided into 2 segments and randomly divided into 2 groups （n =20 each）：control group and IL-1 group.In IL-1 group,the thoracic aortic rings were incubated with Kreb solution containing 20 ng/ml IL-1 for 2 h,and contracted with cumulative concentrations of phenylephrine,ranging from 10-9 to 10-5mol/L.In control group,the thoracic aortic rings were incubated with Kreb solution for 2 h,and contracted with cumulative concentrations of phenylephrine,ranging from 10 9 to 10-5mol/L.Part Ⅱ The thoracic aortic rings were divided into 3 segments and randomly divided into 3 groups （n=20 each）：IL-1 group,IL-1＋ L-NAME （the NOS inhibitor） group and IL-1 ＋cyclooxygenase inhibitor indomethacin group （IL-1 ＋Ⅰgroup）.The thoracic aortic rings were incubated with Kreb solution containing 20 ng/ml IL-1 for 1.5 h in the three groups.In addition,in IL-1 ＋L-NAME and IL-1 ＋Ⅰ groups,the thoracic aortic rings were incubated for 30 min with Kreb solution containing 100 μmol/L L-NAME and 2.5 mmol/L indomethacin,respectively.Contraction of the thoracic aorta was then induced with cumulative concentrations of phenylephrine,ranging from 10 9 to 10-5 mol/L.In group IL-1,the thoracic aortic rings were incubated with Kreb solution.The maximum contractile tension of the thoracic aortic rings was recorded at each concentration of phenylephrine,and the percentage of the maximum contractile tension at the concentration of 10-6 mol/L in group C was obtained.Results Part Ⅰ The percentage of contractile tension at phenylephrine 10-s,10-7,l0 6 and 10-5mol/L was significantly decreased in IL-1 group as compared with C group.Part Ⅱ The percentage of contractile tension at phenylephrine 10-7,10-6 and 10-5mol/L was signific

关 键 词：白细胞介素1 胸主动脉 一氧化氮 前列环素 

分 类 号：R543.1[医药卫生—心血管疾病]