调节趋化因子CXCL12、CCL2、RANTES及其受体表达对自然流产模型小鼠妊娠结局的影响  

Effects of regulation of expressions of chemokines-CXCL12,CCL2,RANTES and their receptors on pregnancy outcome of mouse model of spontaneous abortion

作　　者：张钰[1] 王建梅[1] 顾艳[1] 李俊霞[1] 李奕[1] 

机构地区：[1]天津医科大学第二医院计划生育科,天津300211

出　　处：《中国妇幼保健》2015年第15期2407-2411,共5页Maternal and Child Health Care of China

基　　金：天津市高校科技发展基金项目〔20120123〕

摘　　要：目的:观察选择性改变母胎界面趋化因子CXCL12、CCL2、RANTES及其受体CXCR4、CCR2、CCR5的表达对自然流产模型小鼠妊娠结局的影响,探讨免疫学改造在不明原因复发性流产(URSA)治疗中的可行性。方法:选择经典模型动物建立正常妊娠与URSA小鼠模型,正常妊娠小鼠为对照组(10例),URSA小鼠随机分为5组:未干预组(10例)、CXCL12干预组(10例)、CCR2拮抗剂干预组(10例)、CCR5拮抗剂干预组(10例)和CXCL12+CCR2拮抗剂+CCR5拮抗剂联合干预组(10例)。Western Blot方法检测各组小鼠绒毛和蜕膜组织中趋化因子及其受体CXCL12/CXCR4、CCL2/CCR2、RANTES/CCR5蛋白的表达;同时观察各组小鼠胚胎丢失的情况,计算胚胎丢失率。结果:1URSA小鼠未干预组绒毛和蜕膜组织中CXCL12/CXCR4蛋白的表达显著低于正常妊娠对照组,而CCL2/CCR2和RANTES/CCR5蛋白的表达均显著高于正常妊娠对照组,差异有统计学意义(P均<0.05)。URSA小鼠CXCL12干预组绒毛和蜕膜组织中CXCL12/CXCR4蛋白的表达显著高于未干预组(P<0.05);但仍低于正常妊娠对照组(P<0.05)。URSA小鼠CCR2拮抗剂干预组绒毛和蜕膜组织中CCL2/CCR2蛋白的表达显著低于未干预组(P<0.05),但仍高于正常妊娠对照组(P<0.05)。URSA小鼠CCR5拮抗剂干预组绒毛和蜕膜组织中RANTES/CCR5蛋白的表达显著低于未干预组(P<0.05),但仍高于正常妊娠对照组(P<0.05)。URSA小鼠CXCL12+CCR2拮抗剂+CCR5拮抗剂联合干预组绒毛和蜕膜组织中CXCL12/CXCR4蛋白的表达显著高于未干预组(P<0.05);但仍低于正常妊娠对照组(P<0.05);CCL2/CCR2和RANTES/CCR5蛋白的表达均显著低于未干预组(P<0.05),但仍高于正常妊娠对照组(P<0.05)。2URSA小鼠未干预组的胚胎吸收率显著高于正常妊娠对照组,差异有统计学意义(P<0.01)。URSA小鼠CXCL12干预组、CCR2拮抗剂干预组、CCR5拮抗剂干预组和CXCL12+CCR2拮抗剂+CCR5拮抗剂联合干预组的胚胎吸收率均低于未干预组(P均<0.05),�Objective： To observe the effects of selective differential regulation of expressions of ehemokines-CXCL12, CCL2, R.ANTES and their receptors-CXCR4, CCR2, CCR5 on pregnancy outcome of mouse model of spontaneous abortion, explore the feasibility of immunological transformation in treatment of unexplained recurrent spontaneous abortion （URSA） . Methods： Normal pregnant mouse model and URSA mouse model were established, 10 normal pregnant mice were included in control group, URSA mice were randomly divid- ed into non-intervention group, CXCL12 intervention group, CCR2-antagonist intervention group, CCRS-antagonist intervention group, and joint intervention group （CXCL12＋CCR2-antagonist＋CCRS,antagonist）, 10 mice in each group. Western Blot was used to detect the ex- pressions of CXCL12/CXCR4, CCL2/CCR2, RANTES/CCR5 proteins in villi and decidua; embryo loss situations of mice in the five groups were observed, the rates of embryo loss were calculated. Results： The expressions of CXCL12/CXCR4 proteins in villi and decidua of non- intervention group were statistically significantly lower than those in control group, while the expressions of CCL2/CCR2 and RANTES/CCR5 proteins in villi and deciduas of non-intervention group were statistically significantly higher than those in control group （ P〈0. 05 ） . The ex- pressions of CXCL12/CXCR4 proteins in villi and decidua of CXCL12 intervention group were statistically significantly higher than those in non-intervention group （P〈0. 05 ）, but they were statistically significantly lower than those in control group （P〈0. 05 ） . The expressions of CCL2/CCR2 proteins in villi and decidua of CCR2-antagonist intervention group were statistically significantly lower than those in non-inter- vention group （P〈0. 05）, but they were statistically significantly higher than those in control group （P〈0. 05） . The expressions of RAN- TES/CCR5 proteins in villi and decidua of CCR5-antagonist intervention group were statistically Significantly low

关 键 词：趋化因子受体基质细胞衍生因子 单核细胞趋化蛋白-1 正常T细胞活化后表达和分泌的调节蛋白 流产 

分 类 号：R714.21[医药卫生—妇产科学]