基于荧光分析法的水体叶绿素a传感器光通路设计  

Optical Path Design of Chlorophyll a Sensor of Water Based on Fluorimetry Method

作　　者：李鑫星[1] 王聪[1,2] 陈英义[1,2] 赵佳[3] 傅泽田[1,4] 张领先[1,2] 

机构地区：[1]中国农业大学信息与电气工程学院,北京100083 [2]农业部农业信息获取技术重点实验室,北京100083 [3]山东省农业科学院科技信息研究所,济南250100 [4]食品质量与安全北京实验室,北京100083

出　　处：《农业机械学报》2015年第5期300-305,共6页Transactions of the Chinese Society for Agricultural Machinery

基　　金：山东省自主创新专项资助项目(2012CX90204)

摘　　要：基于荧光分析法,设计了一种水体叶绿素a传感器,可满足长期海洋、湖泊环境实时检测的要求。阐述了荧光分析法测量水体叶绿素a的基本原理,提出了叶绿素a传感器光通路部分的设计方法：首先设计了光学传感器探头;进而基于叶绿素a在430～470 nm蓝光波段吸收作用最强的特性,选用460 nm波长的超高亮蓝色发光二极管作为荧光激励信号设计传感器的信号源,激励光源选用4组超高亮蓝色发光LED,符合叶绿素a强吸收定光谱特性,具有功耗小、体积小、成本低和电路设计简单等优点。对比分析了几种不同光电转化器件的优缺点,选择光敏二极管作为光接收器件,满足了水体叶绿素a荧光信号微弱的检测特点;最后对传感器进行了标定与校准。结果表明,传感器测量结果决定系数R2为0.998,标准偏差为0.05μg,最低检出限为0.25μg/L。The sensor of chlorophyll a based on fluorimetry was designed. It can satisfy the long-term and real-time requirement of sea and lake detection. Firstly, this paper introduces the basic principle of fluorimetry＇ s detecting chlorophyll a based on the former research. The fluorimetry sensor belongs to optical sensor. Optical path design, the key part of hardware, was described as follows： first of all, the optical sensor probe was designed; then the 460 nm super bright blue light diode was selected as the fluorescence excitation signal source, because chlorophyll a has the strongest absorption in 430 -470 nm blue brand. Four sets of super bright blue light emitting LED were used, which suited the characteristic of chlorophyll a＇ s absorption and had the advantages of low power consumption, small volume, low cost and simple circuit design. For the photosensitive receiver module, there are advantages and disadvantages of different electric converters. Finally, the light-sensitive diode was chosen, as it satisfied the requirement of weak fluorescent signal of chlorophyll a. The sensor was calibrated at last. This paper built the standard curve of sensor and the standard curve based on the direct fluorescent method, the correlation coefficient of sensor meansurement is 0. 998, standard deviation is 0. 05μg, and limit of detection is 0. 25μg/L.

关 键 词：水体叶绿素A 荧光分析法 传感器 光通路 设计 

分 类 号：S2[农业科学—农业工程]