基于元分析的大豆含硫氨基酸相关基因挖掘与信息学分析  

Mining and analysis of genes related to sulfur- containing amino acids in soybean based on Meta- QTL

作　　者：邱红梅[1,2] 厉志[2] 于妍 高淑芹[2] 马晓萍[2] 郑宇宏[2] 孟凡凡[2] 侯云龙[2] 王跃强[2] 王曙明[2] 

机构地区：[1]东北农业大学大豆生物学教育部重点实验室,黑龙江哈尔滨150030 [2]吉林省农业科学院大豆研究所/大豆国家工程研究中心,吉林长春130033 [3]长春科技学院,吉林长春130600

出　　处：《中国油料作物学报》2015年第2期141-147,共7页Chinese Journal of Oil Crop Sciences

基　　金：东北农业大学大豆生物学教育部重点实验室开放基金(SB12A02);国家高技术研究发展计划"863计划"(2012AA101106);国家自然科学基金(31401404);吉林省自然科学基金(20140101142JC)

摘　　要：提高含硫氨基酸组分是大豆品质改良的重要目标之一。本文借助Consensus Map 4.0高密度大豆遗传图谱,整合了33个含硫氨基酸QTL,并采用元分析方法,确定了8个含硫氨基酸Meta-QTL。通过QTL序列共线性区间分析,在Meta-QTL内筛选到7条用于候选基因发掘的基因组序列。利用基因功能注释,得到16个含硫氨基酸相关基因,包括6个氨基酸合成途径酶基因、1个11S大豆球蛋白A5A4B3亚基基因及9个调控基因。其中调控基因分别编码F-Box、PPR及转录因子3种蛋白。13个基因在NCBI数据库中分别有表达谱及SNP信息。大豆含硫氨基酸候选基因分析,可以为功能标记的开发和分子辅助育种提供有用信息。Increasing the sulfur- containing amino acid content has been one of the most important goals to improve soybean quality. The objectives of this paper were to discover the soybean sulfur- containing amino acid Meta- QTLs and candidate genes related to sulfur- containing amino acid components in order to establish a basis of developing functional markers for the sulfur- containing amino acids. 33 QTLs related to sulfur- containing amino acids in soybean were integrated by using the reference map of Consensus Map 4. 0 high density soybean genetic map. By Meta- analysis method,8 Meta- QTLs for sulfur- containing amino acids were estimated. 7 long sequences used for screening candidate genes were identified in Meta – QTLs,by collinear interval of QTL sequence. 16 sulfur- containing amino acid related genes were acquired by using function annotation of genes,including 6 genes of amino acid synthetic enzyme,1 gene of 11 s glycinin A5A4B3 and 9 regulated genes. Regulated genes respectively encoded F- Box protein,PPR protein and transcription factors. There were EST profiles and SNPs of 13 genes in NCBI database. Analysis of candidate genes related to sulfur- containing amino acids in soybean could provide useful information for molecular marker assisted breeding.

关 键 词：大豆 含硫氨基酸 元分析 Meta-QTL 候选基因 

分 类 号：S565.103[农业科学—作物学]