抗稻瘟病 Pi2/9/z-t 基因特异性分子标记的开发  

Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t

作　　者：华丽霞[1,2] 汪文娟[1,2] 陈深[1,2] 汪聪颖[1,2] 曾烈先 杨健源[1] 朱小源[1] 苏菁[1,2] 

机构地区：[1]广东省农业科学院 植物保护研究所,广州510640 [2]广东省植物保护新技术重点实验室,广州510640

出　　处：《中国水稻科学》2015年第3期305-310,共6页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31301304,31101403);国家科技(转基因)重大专项(2014ZX0800904B);公益性行业科研专项(201203014);广东省自然科学基金资助项目(S2012010010018);国家重点实验室开放课题资助项目(SKL-CUSAb-2013-10);现代农业产业技术体系建设专项(CARS-01-24,粤财教[2009]356号)

摘　　要：通过对已克隆的抗稻瘟病基因Pi2、Pi9以及Piz-t进行序列比对,寻找各自特异的核苷酸差异,成功开发了基于PCR技术以及电泳检测技术的Pi2/Pi9/Piz-t以及Piz-t的基因特异性分子标记,能有效地将Pi2/Pi9/Piz-t与该位点上的其他抗性等位基因及感病等位基因区分开,这为分子标记辅助育种及抗病基因聚合提供有效的分子标记。用Pi2/Pi9/Pizt基因特异性分子标记对来自全国各稻区的共101份水稻品种和育种亲本进行分子检测,结果发现,除2个品种检测到Piz-t带型之外,大部分水稻品种不携带这3个抗性基因,这为有目的地开展品种的抗性改良提供了重要的参考信息。Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t ,designated as Pi2SNP,Pi9SNP and Pizt-PA,respectively,have been successfully developed through sequence comparison method.These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus,which facilitate the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R genes. Additionally,101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers,and the results showed that only 2 out of 101 cultivars carried Piz-t ,but neither Pi2 nor Pi9 band had been detected.So far,it indicated that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Piz-t .These results had provided important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.

关 键 词：水稻 稻瘟病 抗性基因 分子标记 分子标记辅助选择育种 Pi2/9位点 

分 类 号：S511[农业科学—作物学]