大鼠骨髓来源树突状细胞的培养及鉴定  

Culture and identification of dendritic cells derived from rat bone marrow cells

作　　者：高凌云[1] 刘景丰[2] 陈丽红[1] 

机构地区：[1]福建医科大学基础医学院病理学系,福州350004 [2]福建省肝病科学研究中心福建医科大学孟超肝胆医院

出　　处：《中华实验外科杂志》2015年第5期1006-1008,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（81202337）;福建省卫生厅中青年骨干基金资助项目（2014-ZQN-JC-25）;福州市科技局计划项目（2013-S-125-9）

摘　　要：目的 鉴定体外诱导培养大鼠树突状细胞（DCs）的形态.方法 利用细胞因子体外诱导和大量扩增大鼠骨髓来源的DCs,并采用苏木素-伊红（HE）染色、巴氏染色和Diff-Quick染色进行形态学鉴定,采用免疫组织化学及免疫荧光染色方法检测细胞表面标志物OX62和CD86分子对DCs进行表型鉴定.结果 DCs表面有突起,胞核不规则,胞质可见吞噬小泡.成熟DCs（mDCs）和不成熟DCs（imDCs）表面均表达OX62分子,imDCs低表达CD86分子,而mDCs表面高表达CD86分子.结论 建立在体外大量扩增和多种鉴定大鼠骨髓来源DCs的方法.Objective To identify the dendritic cells （DCs） induced and cultured by rat bone marrow stems in vitro.Methods Dendritic cells derived from rat bone marrow were induced and propagated in vitro.DCs were confirmed morphologically using H＆amp;E staining,Pap staining and Diff-Quick staining.Two surface markers for OX62 and CD86 were detected by immunohistochemistry,and immunofluorescence methods.Results DCs showed irregular shapes and nuclei with phagocytosed vesicules in the cytoplasm.Both mDCs and imDCs expressed OX62.CD86 was low expressed in imDCs,but highly expressed in mDCs.Conclusion We have established valid methods for amplification and identification of DCs.

关 键 词：骨髓 树突状细胞 细胞培养 鉴定 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]