草酸钙结石患儿的肠道产甲酸草酸杆菌与尿草酸的关系  

Intestinal colonization with oxalobacter formigenes and its relation to urinary oxalate excretion in pediatric urolithiasis with idiopathic calcium oxalate

作　　者：江涛[1] 毛厚平[1] 吕夷松[1] 周辉良[1] 陈沁[1] 曹林升[1] 袁华[1] 林熹[1] 高锐[1] 

机构地区：[1]福建医科大学附属第一医院泌尿外科,福州350005

出　　处：《中华实验外科杂志》2015年第5期1179-1180,共2页Chinese Journal of Experimental Surgery

基　　金：福建省高校教育校级立项基金项目（J12015）

摘　　要：目的 探讨特发性草酸钙结石患儿的肠内产甲酸草酸杆菌的定殖以及与24h尿草酸的关系.方法 应用聚合酶链反应（PCR）检测20例特发性草酸钙结石患儿（2～18岁）的大便样本中的产甲酸草酸杆菌的存在.根据年龄、性别匹配20名健康儿童作为对照组.同时,留取这些儿童的24h尿并使用离子色谱法测定尿草酸含量.结果 在结石组中,6例检出产甲酸草酸杆菌（30％）.与正常对照组比较,其定殖率差异无统计学意义（40％,P＞0.05）.在结石组中,检测到肠道定殖产甲酸草酸杆菌的患儿的24 h尿草酸排泄要明显低于未检测到定殖的患儿[（0.79±0.29） mmol/（kg·24 h）比（1.16 ±0.32） mmol/（kg·24 h）,P＜0.05].结论 特发性草酸钙结石患儿的高草酸尿排泄可能是因为肠道内产甲酸草酸杆菌缺乏导致.Objective To study the colonization with oxalobacter formigenes in pediatric urolithiasis and the relationship with 24 h urinary oxalate excretion.Methods The presence of oxalobacter formigenes stool samples of 20 children and adolescents （aged 2-18 years） with idiopathic calcium urolithiasis was assessed using polymerase chain reaction （PCR） method.Twenty healthy,age-and sex-matched subjects served as controls.Simultaneously,urinary oxalate excretion was measured by ion chromatography in this group.Resuts Oxalobacter formigenes was found in 6/20 patients （30%）.In controls,frequency of colonization had no statistically significant difference （40%,P 〉0.05）.The 24 h urinary oxalate excretion in patients colonized with oxalobacter formigenes was significandy lower than in non-colonized patients [（0.79 ± 0.29） mmol/（kg·24 h） vs.（1.16 ± 0.32） mmol/（kg· 24 h）,P 〈 0.05].Conclusion Higher urinary oxalate excretion in children with calcium urolithiasis may be a result of the absence of oxalobacter formigenes.

关 键 词：产甲酸草酸杆菌 特发性草酸钙结石 儿童 

分 类 号：R726.9[医药卫生—儿科]