无辅助呼吸大鼠心肌梗死模型的制备  

To develop a rat model of myocardial infarction without assistant ventilated

作　　者：韩克[1] 刘昱[1] 李涛[2] 马爱群[1] 董安平[1] 吴格如[1] 席羽涛 

机构地区：[1]西安交通大学医学院第一附属医院心内科.教育部环境与疾病相关基因重点实验室 [2]西安市中心医院心内科

出　　处：《陕西医学杂志》2015年第5期515-517,共3页Shaanxi Medical Journal

基　　金：陕西省科技攻关项目(2012K15-01-01)

摘　　要：目的:建立简单、有效的大鼠心肌梗死模型,不用气管插管和呼吸机,直接开胸,在直视下结扎冠状动脉,制备大鼠心肌梗死模型。方法:不用气管插管和呼吸机,经胸骨左缘切口开胸,保持胸膜腔完整,缝扎左室支。术后6周,做病理检查,明确梗死存在并测量梗死面积。结果:大鼠围手术期死亡率23.1%,与假手术组相比,6周后冠脉结扎组左室增大,左室收缩舒张功能受损,病理检查示心肌梗死存在,梗死面积平均为26.6%,两组大鼠心肌梗死面积有显著性差异(P<0.05)。结论:本法成功建立了大鼠心肌梗死模型,具有简便、实用、成功率高之特点;大鼠可作为有关心肌梗死临床研究模型所用合适动物。Objective ：To develop a new model of myocardial infarction The rat was not intubated and not ventilated using a animal ventilator .Methods ：The rat was not intubated and not ventilated using a animal ventila‐tor .A thoracotomy was performed along left sternum with pleura cavity integrity .The major ventricular branch of the left coronary artery was ligated 1‐2mm under the left atria .Sham‐operated rats served as controls .Results ：Perioperative mortality of rats with myocardial infarction was 23 .1% .Six weeks after ,echocardiography suggests that the coronary ligation groups had significantly more dilated left ventricles with lower EF 、FS than the control group（P〈 0 .05） .The infarcted area in the coronary ligation animals was nearly always transmural ,the total infarct area ranged from 19 to 38% and there were significant correlations between infarct size and left ventricular impair‐ment as assessed by ejection fraction ,left ventricular dimension （P 〈 0 .05） .Conclusion ：We constituted a simple , rapid ,and effective method of producing myocardial infarction in rat ;rat can be used as a appropriate myocardial in‐farction model in clinical reaserch .

关 键 词：心肌梗塞/病理学 心肌梗塞/治疗 模型 动物 大鼠 @无辅助呼吸 

分 类 号：R542.22[医药卫生—心血管疾病]