醒脑静注射液对脓毒症大鼠心肌线粒体氧化应激损伤的作用  

Effect of Xingnaojing injection in myocardial mitochondrial oxidative stress injury in sepsis mice

作　　者：王金华[1] 车頔[1] 陈东[2] 曾其毅[1] 

机构地区：[1]南方医科大学附属珠江医院儿科,广州市510282 [2]南方医科大学附属南方医院,广州市510515

出　　处：《实用医学杂志》2015年第9期1403-1406,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81272070)

摘　　要：目的:观察醒脑静注射液对脓毒症大鼠心肌线粒体氧化应激的影响。方法:以腹腔注射脂多糖(LPS)建立脓毒症模型,胃管注入醒脑静,将70只SD大鼠随机分为7组,每组10只:脓毒症6 h组(LPS 6h组)、LPS 24 h组、LPS 48 h组、醒脑静治疗6 h组(XNJ 6 h组)、XNJ 24 h组、XNJ 48 h组、对照组。观察各组不同时点心肌线粒体电镜下形态改变、半定量评分和超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、一氮化氮合酶(i NOS)水平的动态变化。结果 :醒脑静注射液可改善脓毒症大鼠心肌线粒体电镜下的形态改变,降低心肌线粒体电镜下的半定量评分,并可显著降低脓毒症大鼠心肌线粒体MDA、NO、i NOS水平和提高脓毒症大鼠心肌线粒体SOD水平。结论:脑静注射液可显著降低脓毒症大鼠心肌线粒体氧化应激的水平和提高清除氧自由基的能力,从而起到保护脓毒症大鼠心肌线粒体的作用。Objective To observe the effect of Xingnaojing injection in myocardial mitochondrial oxidative stress injury in sepsis mice. Methods The septic model were set up by receiving endotoxin through interaperitoneal injection. After Xingnaojing injection by gastric tube , seventy mice were randomly divided into 7 groups in ten of each group including group LPS-6 h , group LPS-24 h , group LPS-48 h; group XNJ-6 h , group XNJ-24 h, group XNJ-48 h and control group. The myocardial mitochondrial changes , the semi-quantitative scores and the level of SOD、MDA、NO、iNOS in sepsis mice were observed. Results Xingnaojing injection could improve the myocardial mitochondrial changes , reduce the semi-quantitative scores , significantly reduce the level of MDA、NO、iNOS and elevate the level of SOD. Conclusion Xingnaojing injection could significantly reduce the myocardial mitochondrial oxidative stress level and improve the ability of clearing oxygen radicals , thereby protect the myocardial mitochondrion in sepsis mice.

关 键 词：脓毒症 醒脑静注射液 超氧化物歧化酶 丙二醛 一氧化氮 一氮化氮合酶 

分 类 号：R459.7[医药卫生—急诊医学]