京海黄鸡肌细胞生成素基因(MyoG)组织表达谱及表达规律  

Tissue Expression Profile and Expression Pattern of Myogenin Gene(MyoG) in Jinghai Yellow Chicken(Gallus gallus)

作　　者：张涛[1] 张跟喜[1] 张向前[1] 王金玉[1] 唐莹[1] 樊庆灿[1] 薛倩[1] 王永娟 

机构地区：[1]扬州大学动物科学与技术学院/动物遗传繁育与分子设计重点实验室,扬州225009 [2]江苏京海集团,南通226103

出　　处：《农业生物技术学报》2015年第5期617-622,共6页Journal of Agricultural Biotechnology

基　　金：江苏省高校自然科学研究(No.12KJB230003);国家肉鸡产业技术体系(No.nycytx-42-G1-05);江苏高校优势学科建设工程;江苏省动物遗传繁育与分子设计重点实验室

摘　　要：肌细胞生成素基因(myogenin,Myo G)是成肌调节因子家族生肌调节因子家族(myogenic regulatory factor family,MRFs)的成员之一,在调节肌肉生成方面发挥了重要的作用。为了探究Myo G的表达谱和表达规律,研究其可能的作用机理,本研究根据前期克隆得到的京海黄鸡(Gallus gallus)Myo G编码序列(coding sequence,CDS),跨内含子设计一对荧光定量引物,采用实时荧光定量PCR技术检测Myo G在不同组织的表达情况,绘制其组织表达谱;并选取4个时间点,检测在高表达肌肉组织中的表达情况,以研究其表达规律。结果显示,Myo G在胸肌和腿肌中高表达,极显著高于其他组织(P<0.01),在心脏和卵巢组织中也有表达,但是表达量较低。Myo G的表达呈不断变化的趋势,在母鸡胸肌和腿肌中表达变化较为平稳,在公鸡胸肌和腿肌中表达变化较大,4周龄表达量最高,0周龄最低;Myo G在公鸡和母鸡中均是胸肌表达量高于腿肌。本研究揭示了京海黄鸡Myo G的表达谱以及随肌肉生长发育的表达规律,为进一步研究Myo G的作用机理和表达调控提供基础资料。Myogenin gene （MyoG） is a member of myogenic regulatory factors （MRFs） family and plays an important role in regelation of muscle building. To explore the expression profile and expression pattem of MyoG gene and its possible mechanism of action, a pair of fluorescence quantitative primers according to the cloned coding sequence （CDS） of MyoG in Jinghai yellow chicken （Callus gallus） was designed. Real-time fluorescent quantitative PCR was used to detect the expression level of MyoG in different tissues to draw the expression profile. Then, the expression levels of MyoG gene in high expression tissue of breast and leg muscle at four time points were detected to study the expression pattem. The results showed that MyoG mainly expressed in breast and leg muscle and the expression level was extremely significant higher （P〈0.01） than that in other tissues. Expression of MyoG was also detected in heart and ovary, however, the expression level was very low. Expression pattem of MyoG showed a change trend. The variation of MyoG expression in breast and leg muscle of female chicken was stable. The expression in breast and leg muscle of male chicken showed significant changes. Expression of MyoG gene achieved the highest level at 4 weeks of age and lowest level at 0 week of age. For male and female chicken, expression level ofMyoG gene in breast muscle was higher than that in leg muscle. This study revealed the expression profile and expression pattern with the growth and development of muscle of Jinghai yellow chicken, and might lay a foundation for further study on mechanism of action and expression regulation ofMyoG gene.

关 键 词：表达谱 表达规律 肌细胞生成素基因(MyoG) 京海黄鸡 

分 类 号：S828.2[农业科学—畜牧学]