18β-甘草次酸对变应性鼻炎大鼠鼻黏膜中CCL11、AQP1和EOS表达的影响  

Effects of 18β-glycyrrhetinic Acid on the Expression of CCL11,AQP1 and EOS in Nasal Mucosa of Allergic Rhinitis Rats

作　　者：李娟丽[1] 席克虎[1] 侯赟[1] 江英[1] 桂岩[1] 王有虎[1] 张福宏[1] 张小兵[1] 

机构地区：[1]兰州大学第一医院耳鼻咽喉头颈外科,兰州730000

出　　处：《四川大学学报（医学版）》2015年第3期389-393,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金项目(No.81160449);甘肃省卫生厅行业科技计划项目(No.GSWST2011-06);甘肃省中医药科学技术研究课题(No.GZK-2011-22)资助

摘　　要：目的观察18β-甘草次酸（glycyrrhetinic acid,GA）对变应性鼻炎（allergic rhinitis,AR）大鼠鼻黏膜中嗜酸粒细胞趋化因子11（CCL11）、水通道蛋白1（AQP1）和嗜酸粒细胞（EOS）表达的影响。方法将Wistar大鼠随机分为生理盐水对照（NC）组、AR模型（AR）组、氯雷他定（LOA）组、18β-GA组。1~14d腹腔注射卵清蛋白（OVA）和Al（OH）3基础致敏,14~21d用OVA鼻腔激发建立AR模型,NC组均以生理盐水替代。第21d始LOA和18β-GA组分别给予氯雷他定和18β-GA每日1次灌胃,NC与AR组以生理盐水替代。干预1、2、3周后比较各组大鼠行为学评分,取大鼠鼻黏膜行实时荧光定量PCR（RT-QPCR）检测CCL11基因、免疫组化（SP法）检测AQP1表达程度,HE染色观察鼻黏膜组织结构和EOS浸润程度。结果 AR组大鼠1、2、3周时均出现典型AR症状,行为学叠加评分〉5分,鼻黏膜中CCL11、AQP1表达和EOS浸润较NC组增强（P〈0.05）;各时间点,LOA组大鼠上述结果均低于AR组（P〈0.05）;18β-GA组大鼠1周时上述各结果与AR组比较下降（P〈0.05）,2周后各结果接近于LOA组、NC组（P〉0.05）。结论 18β-GA干预可降低CCL11、AQP1的表达水平及EOS浸润程度,抑制AR进展。Objective To investigate the effects of 18β-glycyrrhetinic acid（GA）on the expression of eotaxin1（CCL11）,aquaporin protein 1（AQP1）and eosinophil（EOS）in nasal mucosa of allergic rhinitis（AR）rats.Methods Seventy six Wistar rats were randomly divided into 4groups,normal control（NC）group,AR model（AR）group,loratadine（LOA）group and 18β-GA group.All the mice in AR,LOA and 18β-GA groups were sensitized intraperitoneally with OVA and AL（OH）3from day 1-14,then induced by intranasal administration with OVA from day 14-21,while the mice in NC group were sensitized with saline.The mice in both LOA and 18β-GA group were given LOA and 18β-GA once a day respectively from the 21 d,while the mice in AR and NC groups were administrated with saline.At the end of 1week,2weeks and 3weeks,the behavioral changes of mice were observed and recorded,the level of CCL11 mRNA was measured by RT-QPCR,and AQP1 expression was investiaged by SP staing.EOS in nasal mucosa was studied with the methods of HE staining.Results Compared with NC group,AR group showed typical AR symptoms.With the treatments,AR symptom scores and the expression levels of CCL11,AQP1 and EOS in nasal mucosa were improved significantly（P〈0.05）.When compared with AR group,the above statistics in LOA group were down-regulated evidently at different points in time（P〈0.05）.At the end of 1week,the above detection results in 18β-GA group were lower than those in AR group（P〈0.05）.At the end of 2weeks,those parameters approached to the levels of LOA and NC group significantly.Conclusion 18β-GA administration could down-regulate the expression levels of CCL11,AQP1 and EOS in nasal mucosa of allergic rhinitis rats and cast effects on inhibiting the progress of AR.

关 键 词：变应性鼻炎 18Β-甘草次酸 嗜酸粒细胞趋化因子1 水通道蛋白1 嗜酸粒细胞 

分 类 号：R285.5[医药卫生—中药学]