果生刺盘孢菌Colletotrichum fructicola群体遗传结构研究  

Population genetic structure of Colletotrichum fructicola

作　　者：朱丹雪[1,2] 周国英[1] 徐建平[1] 刘君昂[1] 李河[1] 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙410004 [2]中南林业科技大学生命科学与技术学院,湖南长沙410004

出　　处：《菌物学报》2015年第3期366-374,共9页Mycosystema

基　　金：国家自然科学基金(31100479)

摘　　要：研究了10个不同地区Colletotrichum fructicola菌株群体遗传结构。145个病菌样品ITS序列可推导出13种单倍体型(haplotype),其中,Haplotype 5为优势单倍型,有127个菌株。湖北随州地理种群与其他种群遗传分化极大,遗传分化指数(FST)为0.14685–0.40385。AMOVA分析显示,种群间和种群内的遗传变异分别占总变异的13%和87%;Mantel测试显示地理距离与遗传分化没有显著的线性关系;该菌经历过种群扩张,群体间存在有效基因流。nrDNA ITS sequences were used to analyze the genetic structure and genetic diversity of Colletotrichum fructicola populations consisting of 145 strains isolated from 10 sites. A total of 13 ITS haplotypes were identified among which the dominant haplotype （haplotype 5） included 127 specimens from the 10 sites. The genetic differentiation index, FST, revealed significant genetic differentiation between Suizhou populations and the others. AMOVA test showed that 13% of genetic differentiation occurred among population and 87% within population. The Mantel test showed no linear-correlation between genetic distance and geographical distance, Mismatch distribution analysis suggested there was population expansion for Colletotrichum fructicola and gene flow was detected between populations

关 键 词：油茶炭疽病 COLLETOTRICHUM fructicola ITS-5.8S序列 种群遗传结构 

分 类 号：Q933[生物学—微生物学]