土施糖类对丛枝菌根真菌侵染率和产孢的影响  

Effects of sugar on colonization and sporulation of arbuscular mycorrhizal fungi in soil condition

作　　者：陈静洁[1,2] 梁林洲[2] 董晓英[2] 李延[1] 沈仁芳[2] 

机构地区：[1]福建农林大学资源与环境学院,福建福州350002 [2]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏南京210008

出　　处：《菌物学报》2015年第3期394-401,共8页Mycosystema

基　　金：973计划课题(2013CB127401);中国科学院知识创新工程重大项目课题(KSCX2-EW-N-08);国家基金面上项目(41371289);国家自然科学青年基金(41001172);江苏省自然科学基金重点项目(BK2010097)

摘　　要：丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)不能进行光合作用,需要宿主植物提供碳水化合物供其完成整个生命周期,添加外源物质调控AMF和宿主植物的关系被认为是一种可行的措施。通过盆栽实验种植番茄,探索土施不同糖类对摩西球囊霉Glomus mosseae的侵染率、产孢能力和功能(宿主植物生长和养分)的影响。结果表明,添加葡萄糖和蔗糖可提高接种了摩西球囊霉的番茄的地上部生物量以及磷、钾吸收量,但对地上部氮吸收量影响不显著;添加麦芽糖和淀粉对地上部生物量及氮磷钾养分吸收量的影响均不显著。添加糖类处理,土壤碱解氮均有下降趋势;土壤速效磷、速效钾随着地上部磷和钾吸收量增加有下降趋势。糖类添加对土壤有机质没有影响。添加不同糖类均提高了AMF的侵染率,其中添加蔗糖处理的侵染率较单独施用摩西球囊霉菌处理增加了114%。单独施用摩西球囊霉菌剂处理土壤孢子数为10个/g,添加葡萄糖和淀粉处理的孢子数均为8个/g,添加蔗糖和麦芽糖处理的孢子数均为11个/g,添加糖类均对AMF产孢无显著影响。Arbuscular mycorrhiza fungi （AMF） entire life cycle due to their inability of photo symbiotic associations between plant roots an necessarily rely on taking up carbohydrate from host plant roots to fulfill their synthesis. Adding exogenous materials is considered as a feasible way to regulate d AMF. A pot experiment was conducted to assess the effects of applying differentkinds of sugars results showed glucose and su on Glomus mosseae colonization, spore .density and subsequently the growth of Lycopersicon esculentum. The the shoot dry weight and the amount of phosphorus （P） and potassium （K） in shoot were increased by applying crose, but no effect was found by applying maltose and starch. No significant effects were detected on the amount of nitrogen （N） in shoot after adding different sugars. The amount of alkali-hydrolyzale nitrogen in soil was decreased with adding of different sugars. Olsen-P and available K in the soil decreased with the increase of uptake amount of P and K in ground part of plant. No significant effect as found on soil organic matter for all treatments. The root colonization of AMF was ncreased by adding different sugars, and had increased by 27% in treatment of sucrose. The application of different kind of sugars had no significant effect on sporulation capacity of AME

关 键 词：摩西球囊霉 番茄 碳源 侵染率 孢子数 

分 类 号：Q949.32[生物学—植物学]